| Literature DB >> 24838967 |
Abstract
Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.Entities:
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Year: 2014 PMID： 24838967 PMCID： PMC4668268 DOI： 10.1007/978-1-4939-0777-9_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745