| Literature DB >> 24837902 |
Andrei V Filatov1, Min Wang2, Wenjing Wang3, Andrei V Perepelov4, Alexander S Shashkov1, Lei Wang2, Yuriy A Knirel1.
Abstract
On mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C6285, the O-polysaccharide was cleaved at residues of 5,7-diacetamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic acid (di-N-acetyllegionaminic acid, Leg5Ac7Ac) in the main chain. The resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, and the following structure of the tetrasaccharide repeating unit of the linear O-polysaccharide was established: →4)-α-d-Galp-(1→4)-α-Legp5Ac7Ac-(2→3)-β-d-Galp-(1→3)-β-d-GalpNAc-(1→ The O-antigen gene cluster of E. cloacae C6285 was sequenced, the gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.Entities:
Keywords: Bacterial polysaccharide structure; Enterobacter cloacae; Legionaminic acid; O-Antigen; O-Antigen gene cluster
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Year: 2014 PMID： 24837902 DOI： 10.1016/j.carres.2014.01.012
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104