Tristan Gauthier1, Pascal Piver2, Nicolas Pichon3, Romain Bibes4, Angelique Guillaudeau5, Alessandro Piccardo6, Francis Pesteil6, Jeremy Tricard6, Emmanuel Gardet6, Marc Laskar6, Fabrice Lalloué4, Pierre Marquet7, Yves Aubard2. 1. Department of Obstetrics and Gynecology, Limoges University Hospital, Limoges, France. Electronic address: tristan.gauthier@chu-limoges.fr. 2. Department of Obstetrics and Gynecology, Limoges University Hospital, Limoges, France. 3. Medico-Surgical Intensive Care Unit, Limoges University Hospital, Limoges, France. 4. EA 3842 Homéostasie Cellulaire et Pathologies, School of Medicine, University of Limoges, Limoges, France. 5. Department of Anatomopathology, Limoges University Hospital, Limoges, France. 6. Department of Vascular Surgery, Limoges University Hospital, Limoges, France. 7. Department of Pharmacology, Toxicology, and Pharmacovigilance (Unité Mixte de Recherche-S850 Institut National de la Santé et de la Recherche Médicale), Limoges University Hospital, Limoges, France.
Abstract
OBJECTIVE: To describe the feasibility of human uterus retrieval after donation after brain death. DESIGN: Single-center, prospective study. SETTING: University hospital. PATIENT(S): Female brain dead donors. INTERVENTION(S): The families of female brain dead donors were informed about consent to uterus donation. A specific organ retrieval procedure was performed. At the end of the procedure the uterus was removed together with the hypogastric vessels, parametria, and vaginal fornix. The tolerance of the uterus to cold ischemia was evaluated with histology and TUNEL reaction up to 24 hours. MAIN OUTCOME MEASURE(S): Rate of uterus donation refusal. RESULT(S): Between August 1, 2012 and July 31, 2013, seven uteri were retrieved from 14 female multiorgan donors. No refusal to uterus donation occurred. Our surgical protocol did not interfere with vital organ retrieval and was readily accepted by the other transplantation teams. The hypogastric vessels could be preserved in all cases but for one vein loss in the first retrieval. Histology studies did not find major morphologic changes after 24 hours of cold ischemia. Apoptosis was rare. CONCLUSION(S): Uterus retrieval could be part of a reproducible multiorgan procurement procedure. Uterus donation seems readily accepted. This preliminary study is a necessary step before any transplantation project.
OBJECTIVE: To describe the feasibility of human uterus retrieval after donation after brain death. DESIGN: Single-center, prospective study. SETTING: University hospital. PATIENT(S): Female brain dead donors. INTERVENTION(S): The families of female brain dead donors were informed about consent to uterus donation. A specific organ retrieval procedure was performed. At the end of the procedure the uterus was removed together with the hypogastric vessels, parametria, and vaginal fornix. The tolerance of the uterus to cold ischemia was evaluated with histology and TUNEL reaction up to 24 hours. MAIN OUTCOME MEASURE(S): Rate of uterus donation refusal. RESULT(S): Between August 1, 2012 and July 31, 2013, seven uteri were retrieved from 14 female multiorgan donors. No refusal to uterus donation occurred. Our surgical protocol did not interfere with vital organ retrieval and was readily accepted by the other transplantation teams. The hypogastric vessels could be preserved in all cases but for one vein loss in the first retrieval. Histology studies did not find major morphologic changes after 24 hours of cold ischemia. Apoptosis was rare. CONCLUSION(S): Uterus retrieval could be part of a reproducible multiorgan procurement procedure. Uterus donation seems readily accepted. This preliminary study is a necessary step before any transplantation project.
Authors: Benjamin P Jones; Abirami Rajamanoharan; Nicola J Williams; Saaliha Vali; Srdjan Saso; Ifigenia Mantrali; Maria Jalmbrant; Meen-Yau Thum; Cesar Diaz-Garcia; Sadaf Ghaem-Maghami; Stephen Wilkinson; Isabel Quiroga; Peter Friend; Joseph Yazbek; J Richard Smith Journal: Transplant Direct Date: 2021-02-18
Authors: Ludivine Dion; Gaëlle Santin; Krystel Nyangoh Timoh; Karim Boudjema; Louise Jacquot Thierry; Tristan Gauthier; Marie Carbonnel; Jean Marc Ayoubi; François Kerbaul; Vincent Lavoue Journal: J Clin Med Date: 2022-01-29 Impact factor: 4.241