Vagal afferent fibers almost exclusively innervate islets in the rat pancreas as demonstrated by anterograde tracing.

Anterograde tracing from the nodose ganglion with wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) was utilized to investigate vagal afferent innervation of the rat pancreas. Labelled afferent fibers were consistently detected in islets in all animals. Only about 10% of islets were labelled even in best cases. In only 5 out of 16 rats, acini and excretory ducts received afferent innervation. Injections into the right nodose ganglion resulted in labelling preferentially within the splenic lobe, whereas injections into the left ganglion labelled fibers predominantly in the duodenal lobe. These results point to a non-random distribution of vagal afferent fibers within the pancreas, and suggest a role for these afferents in the regulation of endocrine pancreatic function.