Lung transplantation in telomerase mutation carriers with pulmonary fibrosis.

Lung transplantation is the only intervention that prolongs survival in idiopathic pulmonary fibrosis (IPF). Telomerase mutations are the most common identifiable genetic cause of IPF, and at times, the telomere defect manifests in extrapulmonary disease such as bone marrow failure. The relevance of this genetic diagnosis for lung transplant management has not been examined. We gathered an international series of telomerase mutation carriers who underwent lung transplant in the U.S.A., Australia and Sweden. The median age at transplant was 52 years. Seven recipients are alive with a median follow-up of 1.9 years (range 6 months to 9 years); one died at 10 months. The most common complications were haematological, with recipients requiring platelet transfusion support (88%) and adjustment of immunosuppressives (100%). Four recipients (50%) required dialysis for tubular injury and calcineurin inhibitor toxicity. These complications occurred at significantly higher rates relative to historic series (p<0.0001). Our observations support the feasibility of lung transplantation in telomerase mutation carriers; however, severe post-transplant complications reflecting the syndromic nature of their disease appear to occur at higher rates. While these findings need to be expanded to other cohorts, caution should be exercised when approaching the transplant evaluation and management of this subset of pulmonary fibrosis patients. © ERS 2014.

Introduction

Idiopathic pulmonary fibrosis (IPF) is progressive and fatal, and lung transplantation is the only therapy that has been shown to prolong survival [1], [2]. Because of recent changes in allocation algorithms, IPF has emerged as the leading indication, accounting for one-third of lung transplant cases [1], [3]–[7]. Even though IPF remains defined by its idiopathic adjective, its most frequent identifiable genetic cause is inherited mutations in the telomerase genes [8]. Loss of function mutations in TERT, the telomerase reverse transcriptase gene, and TR (also known as TERC), the telomerase RNA, underlie up to 20% and 3% of familial and sporadic pulmonary fibrosis cases, respectively [8], [9]. Mutations in the telomerase accessory component, DKC1, the dyskeratosis congenita 1 gene, can also manifest as IPF, underscoring the important role of telomere dysfunction in IPF pathogenesis [10]. The significance of the genetic diagnosis of telomerase-mediated pulmonary fibrosis to lung transplant management has not been examined.

Mutations in telomerase cause its loss of function and mediate disease through abnormally shortened telomeres [11]. As such, the mutant gene and mutation type are not the primary mediators of disease severity but the telomere length defect, as previously reviewed [11]. Telomeres are DNA–protein structures that protect chromosome ends. With cell division and advancing age, telomeres shorten, and dysfunctional telomeres provoke cellular senescence and apoptosis [11]. IPF patients with telomerase mutations may show age-related cosmetic features such as premature hair greying, but these findings are not specific, and the diagnosis is confirmed by genetic and molecular testing [8]. Although lung disease is the predominant life-threatening manifestation in adults with telomerase mutations, the telomere defect is systemic, and can be recognised in extrapulmonary manifestations that follow a stereotypical, syndromic pattern [12]–[14]. Bone marrow failure is the most frequent extrapulmonary manifestation and is often first detected as isolated thrombocytopenia in the setting of a hypocellular bone marrow [12]–[14]. Patients with telomere disorders are also prone to developing liver cirrhosis, enteropathies and osteoporosis, and have a higher incidence of skin and haematological malignancies [11], [15], [16]. Even in the absence of telomerase mutations, IPF patients can have abnormally short telomeres [17], [18] and can manifest syndromic features of telomere-mediated disease [18]. These observations have suggested telomere defects may be relevant to pathogenesis even beyond the IPF subset with telomerase mutations [18].

We sought here to examine the relevance of the telomere syndrome diagnosis in IPF patients who underwent lung transplantation. This question is of particular significance because bone marrow failure patients with telomere syndromes are exquisitely sensitive to standard bone marrow transplant conditioning regimen, as previously reviewed [19], [20]. These observations have contributed to the current standard of care practice of reducing the intensity of the bone marrow transplant conditioning regimens with significantly improved short-term outcomes [8]. Therefore, pre-transplant screening for telomere syndromes in patients with bone marrow failure has been advocated and is being integrated into standard work-ups since it significantly alters the bone marrow transplant approach [8]. In this study, we report a first and international series of lung transplant experience in pulmonary fibrosis patients who carry mutant telomerase genes. We show that adult telomerase mutation carriers with IPF who undergo lung transplant develop recurrent and otherwise unusual complications that reflect the syndromic nature of their disease. Our observations suggest that recognising these patients, clinically and through genetic evaluation, may inform management in this subset of lung transplant recipients.

Subjects and methods

Subjects were eligible for this study if they carried a mutation in telomerase, or had the diagnosis of a telomere syndrome and had received a lung transplant. Telomere syndrome criteria were defined as previously, and confirmed by abnormally short age-adjusted telomere length [13], [14], [18]. Cases were identified through genetic evaluation at Johns Hopkins, Baltimore, MD, USA and through a collaborative effort of a list-serve for lung transplant physicians from around the world. Extracted and primary medical records were reviewed by two of the authors. The study was approved by the Johns Hopkins Medicine institutional review board, and the human research and ethics committees of contributing institutions. Subjects recruited within the USA gave written, informed consent. Follow-up was updated on March 1, 2014.

To determine the incidence of medication-related toxicities in telomerase mutation carriers, we reviewed the Johns Hopkins experience and the literature for adverse events of commonly prescribed drugs in the lung transplant setting: azathioprine, calcineurin inhibitors, de novo purine synthesis antagonists and antibiotics. Events recorded from five additional pulmonary fibrosis cases enrolled in the Johns Hopkins Telomere Syndrome Registry who received at least one of these medication classes in other settings along with events extracted from a literature review of telomerase mutation carriers with pulmonary disease are included. Details of the manual literature review (through December 31, 2012) have been previously published [15]. We used GraphPad Prism software for statistical analyses (San Diego, CA, USA). The p-values shown are all two-sided.

Results

Lung transplant recipients have clinical features of a telomere syndrome

The eight subjects were transplanted at four centres from 2004 to 2013 in the USA (n=5), Australia (n=2), and Sweden (n=1). The median age at pulmonary fibrosis diagnosis was 47 years (range 42–61 years) and 50% were male. The median age at transplant was 52 years (range 44–64 years). Most subjects showed features of a telomere syndrome prior to transplant, including premature hair greying prior to 25 years of age (six of seven; 86%) and abnormally low blood counts with at least one haematopoietic lineage affected (thrombocytopenia most common, five of eight; 63%). One subject carried the diagnosis of myelodysplastic syndrome, and one had bone marrow failure. Three subjects (38%) had history of resection of squamous or basal cell skin carcinomas. All subjects with available family histories reported having at least one relative with pulmonary fibrosis (six of six; 100%). All subjects had documented normal renal function prior to transplant. The pre-transplant clinical characteristics are summarised in table 1.

Table 1–

Pre-transplant clinical characteristics of telomere patients who received a lung transplant

Age at transplant in years	Age at diagnosis in years	Sex	Genetic diagnosis	Family history	Pathology	Smoking history	Telomere syndrome features/comorbidities	Blood counts			Renal function#
								WBC ×103 mm−3	Hb g·dL−1	Platelets ×103 mm−3
44	44	F	TERT Arg756Cys CGT>TGT	Pulmonary fibrosis	IPF/UIP	Never	Grey, 14 years Coronary artery disease	8.5	11.6↓	123↓	Normal
47	44	M	TERT Val170Met GTG>ATG	Pulmonary fibrosis	IPF/UIP	Never	Grey, 20–30 years Squamous and basal cell carcinomas¶	4.2↓	10.6↓	150	Normal
47	42	F	TERT Ala678Asp GCC>GAC	Not available; adopted	IPF/UIP	Never	Grey, 17 years Bone marrow failure	5.9	12.2	105↓	Normal
49	44	M	TERT Arg743Trp AGG>TGG	Not available	UIP/DIP	Never	Premature greying Coronary artery disease Myelodysplastic syndrome	1.4↓	11.8↓	43↓	Normal
55	50	F	TR 35C>A	Pulmonary fibrosis	UIP/NSIP	Never	Grey, 35 years Squamous cell carcinomas¶	6.9	12.2	152	Normal
61	57	M	TERT Leu841Phe CTC>TTC	Pulmonary fibrosis	IPF/UIP	10 pack-years	Grey, 20–30 years ↑ Liver function tests	8.8	14.2	186	Normal
62	61	F	TR 182G>C	Pulmonary fibrosis Avascular necrosis	IPF/UIP	Never	Grey, 22 years Vertebral compression fracture-osteoporosis Avascular necrosis Basal cell carcinomas	4.9	10.0↓	100↓	Normal
64	58	M	Telomere syndrome; clinical with very short telomeres	Pulmonary fibrosis	IPF/UIP	Never	Grey, 16 years Basal cell carcinomas	14.2	15.3	121↓	Normal

WBC: white blood cell; Hb: haemoglobin; F: female; M: male; IPF: idiopathic pulmonary fibrosis; UIP: usual interstitial pneumonia; DIP: desquamative interstitial pneumonia; NSIP: non-specific interstitial pneumonitis. #: serum creatinine clearance >70 cm3 per minute; ¶: some of these skin cancers were diagnosed post-transplant.

Molecular studies support the telomere syndrome diagnosis

The genetic diagnosis was documented prior to transplant in half the cases. Five subjects carried mutations in TERT, two subjects had mutations in TR, and one fulfilled clinical telomere syndrome criteria with abnormally short telomere length, a positive family history of pulmonary fibrosis, a personal history of thrombocytopenia but no detectable mutation in TERT, TR or DKC1. The TERT and TR mutations identified were absent in large series of controls (n=1500 including the 1000 Genome Project [21]) and fell in highly conserved motifs (fig. 1 and supplementary fig. S1). Four of the mutations were previously reported in telomere disorders or shown to functionally decrease telomerase activity [14], [22], [23]. Where available (five of five), telomere length by flow cytometry and FISH fell below the age-adjusted first percentile, supporting the pathogenic nature of these mutations (fig. 2a). Since the majority of the cases in this series (88%) carry telomerase mutations, similar to the documented literature [14], we will use the terminology telomerase mutation carriers to refer to this cohort hereafter.

Figure 1–

Telomerase mutations in lung transplant subjects fall in conserved domains of telomerase reverse transcriptase (TERT) and telomerase RNA (TR). a) Organisation of conserved reverse transcriptase motifs (boxed) with mutations indicated above (n=5). b) TR mutations fall in the P1b and P3 helices within the secondary structure and are near the template-containing pseudoknot domain. Both are predicted to disrupt Watson-Crick base pairing and thus TR stability.

Figure 2–

Post-transplant complications of telomere syndrome cases. a) Telomere length by flow cytometry and fluorescence in situ hybridisation shows significant shortening relative to age-matched controls (TERT, n=2; TR, n=2; clinical telomere syndrome n=1). The telomere length nomogram is based on 400 controls. b) Pre-transplant platelet counts show a majority of patients had thrombocytopenia (<150×103 platelets mm−3). Post-transplant, platelet counts drop further and in several cases into a range that increases the risk of bleeding (<50×103 platelets mm−3). Post-transplant nadir is defined as within the first two weeks. c) Computed tomography image showing cavitary left lung lesion in a patient with pulmonary aspergillosis at 9 months post-transplant. d) Lung histopathology from autopsy in a subject who died from complications of cytomegalovirus pneumonitis. The photomicrograph shows evidence of diffuse alveolar haemorrhage and multiple viral intranuclear inclusions. e and f) Renal biopsies of two individuals who required prolonged dialysis showing tubular irregularity and epithelial cytoplasmic vacuolisation; these findings are highly consistent with calcineurin inhibitor toxicitiy. Biopsies were obtained at 4 months (e) and 10 months (f) post-dialysis, the latter at the time of autopsy. g) Colon biopsy from individual who developed ischaemic colitis on mycophenolate shows extensive puss, crypt dropout and apoptosis. h) Colon biopsy from the same individual 5 weeks after mycophenolate was discontinued shows resolution of the puss, but residual crypt dropout and epithelial apoptosis similar to histological findings described in other telomere syndrome cases and consistent with an underlying telomere-mediated enteropathy.

Lung transplant outcomes in telomerase mutation carriers

Seven of the eight subjects (88%) are alive with a median follow-up of 1.9 years (range 6 months to 9 years). One subject died 10 months post-transplant after a prolonged hospitalisation. The post-transplant clinical course is summarised in table 2 and in figure 2b–h. Explanted lung pathology documented usual interstitial pneumonia changes in all cases. All the subjects underwent at least two transbronchial biopsies post-transplant. Four had no rejection and four had evidence of minimal rejection (grade A1 B0, 50%, International Society for Heart and Lung Transplantation classification [24]), and one of these had transient evidence of moderate rejection (grade A2) rejection. Several subjects suffered infectious complications with Gram negative pneumonia/sepsis and opportunistic infections, such as pulmonary aspergillosis and cytomegalovirus pneumonitis (fig. 2c and d). One subject had defective healing at the anastomotic site bilaterally with each of sequential lung transplants; delayed wound healing has been reported in other telomere syndrome cases [10].

Table 2–

Post-transplant complications of lung transplant recipients with telomere disorders

Age at transplant in years	Sex	Follow-up	Lung transplant	Respiratory and allograft function	Haematological complications (nadir platelet count ×103 mm−3)	Immunosuppressive dose reduction	Renal failure	Gastrointestinal and other complications
44	F	6 months	Double	Pseudomonas pneumonia and sepsis No rejection	Platelet transfusion (53) Bone marrow biopsy performed	Yes	Acute renal failure/ATN RRT for 2 months Chronic renal insufficiency residual (GFR 33 cm3·min−1)	Critical care illness, severe-polyneuropathy/myopathy Cerebrovascular accident Prolonged hospitalisation (4 months)
47	M	5 years	Double	Minimal rejection (A1B0)	No platelet transfusion (61) Bone marrow biopsy performed	Yes		Gastrointestinal bleed, upper Pyloric ulcer
47	F	11 months	Double	Pseudomonas pneumonia Parainfluenza pneumonitis Minimal rejection (A1B0)	Platelet transfusion (44) PRBC and platelet transfusion-dependent Haemolytic anaemia	Yes	Acute renal failure/ATN Calcineurin inhibitor toxicity RRT, day 5 to 10 months Renal cortical necrosis	Compensated liver cirrhosis Gastrointestinal bleed, upper Gastroduodenal erosions Prolonged hospitalisation (6 months)
49	M	1.9 years	Double	No rejection	Platelet transfusion (22)	Yes	Acute renal failure/ATN RRT for 8 days Chronic renal insufficiency-residual (GFR 30 cm3·min−1)	Critical care illness, polyneuropathy/myopathy
55	F	9 years	Double	Multiple bacterial pneumonias Airway stenosis requiring dilation Minimal and mild rejection (A1, A2)	Platelet transfusion Transfusion dependent at 9 years	Yes	Chronic renal insufficiency evolving to end stage renal disease 9 years post-transplant	Prolonged mechanical ventilation (6 months), diaphragmatic weakness Prolonged hospitalisation (7 months)
61	M	Died 10 months	Single, R Single, L (8 months)	Anastomosis-ischaemia, R Anastomosis-leak with, broncho- pleural fistula, L Enterococcus pneumonia Cytomegaloviral pneumonitis No rejection	Platelet transfusion (25)	Yes	Acute renal failure/ATN Calcineurin inhibitor toxicity RRT, day 24 to transplant Renal transplant, 8 months	Ischemic colitis (MMF-induced) Elevated liver function tests Critical care illness, severe-polyneuropathy/myopathy Prolonged hospitalisation (10 months)
62	F	1.9 years	Single, R Single, L (3 months)	Klebsiella pneumonia and sepsis No rejection	Platelet transfusion (28)	Yes		Elevated liver function tests Pulmonary embolism on erythropoietin
64	M	1.1 years	Single, R	Pulmonary aspergilllosis Minimal rejection (A1 B0)	Platelet transfusion (21) Bone marrow biopsy performed	Yes

F: female; M: male; ATN: acute tubular necrosis; RRT: renal replacement therapy; GFR: glomerular filtration rate; PRBC: packed red blood cells; R: right; L: left; MMF: mycophenolate.

Increased rate of haematological complications post-transplant

Haematological complications were the most prevalent morbidity and required significant adjustment of the immunosuppressive regimen in all cases (100%). Most subjects were able to tolerate only two drug immunosuppressive regimen. A majority of recipients required transfusion support for thrombocytopenia (seven of eight, 88%; fig. 2b), and several required bone marrow evaluation and formal haematology consultation for concerns regarding the severity of thrombocytopenia and risk of bleeding. In a large historical series of 169 patients, a 12% platelet transfusion rate was documented after lung transplantation [25]; therefore, the transfusion need in this international cohort of short telomere patients appears significantly increased (p<0.0001; Chi-squared test). Platelet counts improved with adjustment of myelosuppressive medications but remained lower than pre-transplant levels, and four subjects, including the two cases that had a bone marrow failure diagnosis prior to lung transplant continue to require platelet transfusion prophylactically prior to invasive procedures. One of these individuals also developed haemolytic anaemia and remains red blood cell transfusion-dependent 9 months after transplant (table 2).

High frequency of renal failure requiring RRT in telomerase mutation carriers

In addition to the haematological complications, there was also a surprisingly high rate of acute renal failure and acute tubular necrosis. Four individuals required renal replacement therapy (RRT) in the first 2 weeks post-transplant (50%). Relative to historical series where RRT is indicated in 0.6%–4.5% in the first month after lung transplant [26], [27], this frequency is unusually high (p<0.0001; Chi-squared test). The renal failure was thought to be multi-factorial related to perioperative haemodynamic changes, infections, and calcineurin inhibitor nephrotoxicity. One subject underwent a renal transplant after 8 months, while the other three recovered after 9 months, 2 months and 8 days of RRT, but all continue to have residual chronic renal insufficiency. In addition, the 9-year survivor has developed end stage renal disease. Renal histopathology from two cases that required prolonged dialysis was available, and showed similar findings of tubular epithelial irregularity, sloughing, and proximal tubule cytoplasmic vacuolisation (fig. 2e and f). These findings are hallmarks of calcineurin inhibitor toxicity [28]. Calcineurin-inhibitor nephrotoxicity occurs in 25% of lung transplant recipients at 1 year, but only 2–4% of cases require RRT, suggesting a higher rate of toxicity in the group of patients we studied (p=0.008) [26], [29].

In addition to haematological and renal complications, three individuals developed gastrointestinal bleeding that required red blood cell transfusion support (three of eight, 37%). In one case, the haemorrhage was caused by mycophenolate-related ischaemic colitis, a very rare complication limited to case reports [30], [31]. Colon biopsy after discontinuation of mycophenolate showed residual crypt loss and epithelial apoptosis similar to histopathological findings documented in telomere-related enteropathy (fig. 2g and h) [15]. In some cases there was impaired functional recovery with three individuals developing critical illness polyneuropathy and myopathy; in two cases, this was deemed severe and contributed to prolonged hospitalisation beyond 3 months.

Patients with telomere defects are at risk for rare medication-related toxicities

In addition to mycophenolate and calcineurin inhibitors, lung transplant recipients are exposed to a number of cytotoxic medications as part of the immunosuppression or anti-microbial prophylaxis regimens. Because there is precedence for increased adverse events with medications in patients with telomere disorders (such as busulfan in the bone marrow transplant setting where it causes fatal pulmonary fibrosis [19]), we extended our search to individuals in the Johns Hopkins Telomere Syndrome Registry and the literature to identify serious toxicities that may also be relevant to lung transplant recipients. We identified adverse events with azathioprine, dapsone and inhaled pentamidine. The two azathioprine-related toxicities manifested as fulminant hepatitis, one of which was fatal [12], [32]. Azathioprine-related fulminant hepatitis occurs in less than one in 10 000 cases [33], making these two reports in a small subset highly noteworthy. We also observed methemoglobinaemia with short-term dapsone use, and a first case to our knowledge of transient hypoglycaemia after aerosolised pentamidine, a complication that rarely occurs even with systemic administration [34], [35].

Discussion

In this international series, we report that lung transplantation for IPF patients with telomerase mutations is feasible, and can at times be associated with reasonable survival. The longest surviving case in our series is nine years post-transplant. Nevertheless, our experience suggests that transplantation in this group of patients requires attention to specific complications that may otherwise be rare in lung transplant recipients and that at times can be severe. These include haematological toxicities with otherwise routinely used medications, and in many cases, at least a short-term requirement for transfusion support for significant cytopenias. Awareness of these potential complications may help anticipate, manage and possibly avert post-transplant morbidities in this IPF subset.

Recipients in our series uniformly required dose modification of immunosuppression for cytopenias, reflecting their underlying limited bone marrow reserves. Platelet transfusion support was also necessary and, in some cases, this need was prolonged. Pre-transplant challenge protocols with cytotoxic drugs are used in some experienced centres to test the robustness of bone marrow reserves, and this may be one approach to identify patients who can tolerate the haematological stress of transplant-related myelosuppressive medications. In our series, one patient who carried the diagnosis of myelodysplastic syndrome and one patient who had bone marrow failure received a lung transplant; the former patient is doing well 1.9 years out from transplant and is transfusion independent, while the latter remained transfusion-dependent 11 months post-transplant. These cases highlight the heterogeneity in the severity of bone marrow failure in telomere syndrome patients and the need for multidisciplinary consultation with haematologists in assessing bone marrow function. The clinical criteria, based on medical and family history, for suspecting the telomere syndrome diagnosis along with considerations for a telomere-focused pre-transplant work-up are summarised in figure 3.

Figure 3–

Clinical considerations for a) suspecting and b) evaluating patients with pulmonary fibrosis who may have telomere syndromes.

Our experience suggests it is possible that nephrotoxic drugs used in the transplant setting may uncover a telomere-related renal vulnerability. Despite the fact that all the recipients had normal renal function pre-transplant, four cases (50%) required RRT and, in a subset of these cases, the need was prolonged. Renal disease is not known to occur de novo in telomere syndromes, and has been reported only once after bone marrow transplantation [36]. Calcineurin inhibitors levels are maintained at higher levels in lung transplant recipients because of the relatively high incidence of allograft rejection [37], [38]; this may contribute to uncovering a telomere-related renal vulnerability. Calcineurin inhibitors are also metabolised by hepatic P450 enzymes [39], and it is possible that patients who have subclinical liver disease are prone to decreased clearance. Indeed, in two cases that required prolonged RRT, there was abnormal liver function and evidence of subclinical cirrhosis radiographically (table 2). It is interesting to note that mice with short telomere are prone to renal tubular apoptosis and have an increased predilection to tubular epithelial injury after challenge [40]. Perioperative renal compromise, along with the nephrotoxic effects of calcineurin inhibitors may thus cause a greater than expected tubular injury which may be difficult to reverse because of the telomere defect. One compelling possibility is that telomere syndrome patients may have a lower frequency of allograft rejection because of an immunosenescence phenotype. This hypothesis will need to be tested prospectively and if supported, it may provide a rationale for modifying the current immunosuppressive regimens for this population. Understanding the spectrum of toxicities associated with calcineurin inhibitors in telomere syndrome patients will also be important in informing the optimal approach to immunosuppression in the lung transplant setting.

Despite the small nature of our series, the concordant findings across four centres from around the world support that this group of patients may warrant specific attention to complications that may otherwise be uncommon in other lung recipient populations, such as cystic fibrosis or pulmonary hypertension. In patients with telomere syndromes who require bone marrow transplantation, the experience of recent decades has led to a significant alteration in the approach to bone marrow transplant with improved short-term outcomes [19], [20]. For example, it is considered standard practice to shield the lungs during radiation exposure and to minimise DNA damaging agents [20]. Therefore, in the context of an existing individualised paradigm for bone marrow transplant in these patients, our observations suggest that a similar tailored lung transplant protocol may be necessary to optimise supportive care for this subset of pulmonary fibrosis patients.

Finally, it is important to note that our series reflects only cases that were deemed eligible for transplantation. Transplant candidates with pulmonary fibrosis and concurrent end organ failure in the bone marrow and liver were frequently deemed ineligible at all of our centres. Moreover, despite the specificity of the IPF–bone marrow failure complex for telomere defects, the majority of IPF patients with telomerase mutations cannot be distinguished clinically because they have disease that appears limited to the lung, and these individuals would not have been selected for genetic evaluation [14], [41], [42]. The spectrum of lung disease pathology associated with telomerase mutations is also broad, and although the IPF/usual interstitial pneumonia phenotype is most common in telomerase mutation carriers, in up to 30% of cases, the clinical picture may reflect another idiopathic interstitial pneumonia, as reviewed elsewhere [43]. Pre-transplant identification may facilitate risk assessment and inform post-transplant management for this subset. Importantly, because the telomere defect is commonly seen in IPF patients even in the absence of telomerase mutations [18], [44], our experience raises the possibility that syndromic defects may contribute to the inferior post-transplant outcomes in IPF relative to other lung transplant recipients. These hypotheses are testable and, if validated, may form the basis of a genetically informed transplant management paradigm for a subset of pulmonary fibrosis patients.