Victoria Maneu1, Agustina Noailles2, Javier Megías3, Violeta Gómez-Vicente2, Núria Carpena3, M Luisa Gil3, Daniel Gozalbo3, Nicolás Cuenca4. 1. Departamento de Óptica, Farmacología y Anatomía, Universidad de Alicante, Alicante, Spain Instituto Teófilo Hernando de I+D del Medicamento, Universidad Autónoma de Madrid, Madrid, Spain. 2. Departamento de Fisiología, Genética y Microbiología, Universidad de Alicante, Alicante, Spain. 3. Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Spain. 4. Departamento de Fisiología, Genética y Microbiología, Universidad de Alicante, Alicante, Spain Instituto multidisciplinar para el estudio del medio Ramón Margalef, Universidad de Alicante, Alicante, Spain.
Abstract
PURPOSE: We determined whether systemic fungal infection could cause activation of retinal microglia and, therefore, could be potentially harmful for patients with retinal degenerative diseases. METHODS: Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by confocal immunofluorescence and flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII, and anti-CD45 antibodies. RESULTS: Systemic fungal infection causes activation of retinal microglia, with phenotypic changes in morphology, surface markers expression, and microglial relocation in retinal layers. CONCLUSIONS: As an excessive or prolonged microglial activation may lead to chronic inflammation with severe pathological side effects, causing or worsening the course of retinal dystrophies, a systemic infection may represent a risk factor to be considered in patients with ocular neurodegenerative diseases, such as diabetic retinopathy, glaucoma, age-related macular degeneration, or retinitis pigmentosa. Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
PURPOSE: We determined whether systemic fungal infection could cause activation of retinal microglia and, therefore, could be potentially harmful for patients with retinal degenerative diseases. METHODS: Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by confocal immunofluorescence and flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII, and anti-CD45 antibodies. RESULTS:Systemic fungal infection causes activation of retinal microglia, with phenotypic changes in morphology, surface markers expression, and microglial relocation in retinal layers. CONCLUSIONS: As an excessive or prolonged microglial activation may lead to chronic inflammation with severe pathological side effects, causing or worsening the course of retinal dystrophies, a systemic infection may represent a risk factor to be considered in patients with ocular neurodegenerative diseases, such as diabetic retinopathy, glaucoma, age-related macular degeneration, or retinitis pigmentosa. Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
Authors: Li Jiang; Fan Xu; Wenjing He; Lifei Chen; Haibin Zhong; Yu Wu; Siming Zeng; Li Li; Min Li Journal: Inflamm Res Date: 2016-03-08 Impact factor: 4.575
Authors: Thomas Blank; Tobias Goldmann; Mirja Koch; Lukas Amann; Christian Schön; Michael Bonin; Shengru Pang; Marco Prinz; Michael Burnet; Johanna E Wagner; Martin Biel; Stylianos Michalakis Journal: Front Immunol Date: 2018-01-05 Impact factor: 7.561