Literature DB >> 24833

Overmethylation of DNAs by the EcoRI methylase.

K L Berkner, W R Folk.   

Abstract

EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI endonuclease activity, and thus is presumably at or near the EcoRI sequences (5') NAATTN.

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Year:  1978        PMID： 24833      PMCID： PMC341993          DOI： 10.1093/nar/5.2.435

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

Review 1.  Selective silencing of eukaryotic DNA.

Authors:  R Sager; R Kitchin
Journal:  Science       Date:  1975-08-08       Impact factor: 47.728

2.  Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA.

Authors:  K L Berkner; W R Folk
Journal:  J Biol Chem       Date:  1977-05-25       Impact factor: 5.157

3.  Sites in the polyoma genome cleaved by restriction endonuclease HindII.

Authors:  W R Folk; B R Fishel; D M Anderson
Journal:  Virology       Date:  1975-03       Impact factor: 3.616

4.  Amplification of a specific region of the polyoma virus genome.

Authors:  B E Griffin; M Fried
Journal:  Nature       Date:  1975-07-17       Impact factor: 49.962

5.  Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate.

Authors:  P H Greene; M S Poonian; A L Nussbaum; L Tobias; D E Garfin; H W Boyer; H M Goodman
Journal:  J Mol Biol       Date:  1975-12-05       Impact factor: 5.469

6.  Specificity of substrate recognition by the EcoRI restriction endonuclease.

Authors:  B Polisky; P Greene; D E Garfin; B J McCarthy; H M Goodman; H W Boyer
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

7.  Polyoma DNA: a physical map.

Authors:  B E Griffin; M Fried; A Cowie
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

8.  Physical identity of the SV40 deoxyribonucleic acid sequence recognized by the Eco RI restriction endonuclease and modification methylase.

Authors:  A Dugaiczyk; J Hedgpeth; H W Boyer; H M Goodman
Journal:  Biochemistry       Date:  1974-01-29       Impact factor: 3.162

9.  DNA nucleotide sequence restricted by the RI endonuclease.

Authors:  J Hedgpeth; H M Goodman; H W Boyer
Journal:  Proc Natl Acad Sci U S A       Date:  1972-11       Impact factor: 11.205

10.  Cleavage of Nonglucosylated Bacteriophage T4 deoxyribonucleic acid by Restriction Endonuclease Eco RI.

Authors:  D A Kaplan; D P Nierlich
Journal:  J Biol Chem       Date:  1975-03-25       Impact factor: 5.157
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  2 in total

1.  Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.

Authors:  J Heitman; P Model
Journal:  EMBO J       Date:  1990-10       Impact factor: 11.598

2.  Fidelity index determination of DNA methyltransferases.

Authors:  Janine G Borgaro; Nicole Benner; Zhenyu Zhu
Journal:  PLoS One       Date:  2013-05-06       Impact factor: 3.240
  2 in total

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