| Literature DB >> 24831971 |
Serge Ostrovidov1, Xuetao Shi1, Ling Zhang1, Xiaobin Liang1, Sang Bok Kim2, Toshinori Fujie3, Murugan Ramalingam4, Mingwei Chen1, Ken Nakajima1, Faten Al-Hazmi5, Hojae Bae6, Adnan Memic7, Ali Khademhosseini8.
Abstract
Engineering functional muscle tissue requires the formation of densely packed, aligned, and mature myotubes. To enhance the formation of aligned myotubes with improved contractibility, we fabricated aligned electrospun gelatin multi-walled carbon nanotubes (MWNTs) hybrid fibers that were used as scaffolds for the growth of myoblasts (C2C12). The MWNTs significantly enhanced myotube formation by improving the mechanical properties of the resulting fibers and upregulated the activation of mechanotransduction related genes. In addition, the fibers enhanced the maturation of the myotubes and the amplitude of the myotube contractions under electrical stimulation (ES). Such hybrid material scaffolds may be useful to direct skeletal muscle cellular organization, improve cellular functionality and tissue formation.Entities:
Keywords: C2C12; Carbon nanotubes; Electrospinning; Gelatin fibers; Myotube
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Year: 2014 PMID： 24831971 DOI： 10.1016/j.biomaterials.2014.04.021
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479