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Literature DB >> 24829244

Multiplex PCR assay for identification of six different Staphylococcus spp. and simultaneous detection of methicillin and mupirocin resistance.

E Campos-Peña¹, E Martín-Nuñez¹, G Pulido-Reyes¹, J Martín-Padrón¹, E Caro-Carrillo¹, J Donate-Correa¹, I Lorenzo-Castrillejo¹, J Alcoba-Flórez², F Machín¹, S Méndez-Alvarez³.

Abstract

We describe a new, efficient, sensitive, and fast single-tube multiple-PCR protocol for the identification of the most clinically significant Staphylococcus spp. and the simultaneous detection of the methicillin and mupirocin resistance loci. The protocol identifies at the species level isolates belonging to S. aureus, S. epidermidis, S. haemolyticus, S. hominis, S. lugdunensis, and S. saprophyticus.

Entities: Chemical Species

Mesh：

Substances：

Year: 2014 PMID： 24829244 PMCID： PMC4097714 DOI： 10.1128/JCM.00918-14

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

20 in total

1. Rapid and accurate identification of human-associated staphylococci by use of multiplex PCR.

Authors: Shintaro Hirotaki; Takashi Sasaki; Kyoko Kuwahara-Arai; Keiichi Hiramatsu
Journal: J Clin Microbiol Date: 2011-08-10 Impact factor: 5.948

2. Development of a rapid PCR assay specific for Staphylococcus saprophyticus and application to direct detection from urine samples.

Authors: F Martineau; F J Picard; C Ménard; P H Roy; M Ouellette; M G Bergeron
Journal: J Clin Microbiol Date: 2000-09 Impact factor: 5.948

3. Staphylococcus aureus- the predominant pathogen in the neonatal ICU of a tertiary care hospital in amritsar, India.

Authors: Poonam Sharma; Parminder Kaur; Aruna Aggarwal
Journal: J Clin Diagn Res Date: 2012-11-02

4. Molecular epidemiology of plasmid-mediated high-level mupirocin resistance in methicillin-resistant Staphylococcus aureus in four Spanish health care settings.

Authors: Eduardo Pérez-Roth; Carmen Potel-Alvarellos; Xiomara Espartero; Lucia Constela-Caramés; Sebastián Méndez-Álvarez; Maximiliano Alvarez-Fernández
Journal: Int J Med Microbiol Date: 2013-03-29 Impact factor: 3.473

5. Multiplex PCR assay to identify methicillin-resistant Staphylococcus haemolyticus.

Authors: Ricardo P Schuenck; Eliezer M Pereira; Natalia L P Iorio; Kátia R N Dos Santos
Journal: FEMS Immunol Med Microbiol Date: 2008-02-22

6. Comprehensive strategy including prophylactic mupirocin to reduce Staphylococcus aureus colonization and infection in high-risk neonates.

Authors: H M Delaney; E Wang; M Melish
Journal: J Perinatol Date: 2012-08-23 Impact factor: 2.521

7. Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance.

Authors: Matthew T G Holden; Edward J Feil; Jodi A Lindsay; Sharon J Peacock; Nicholas P J Day; Mark C Enright; Tim J Foster; Catrin E Moore; Laurence Hurst; Rebecca Atkin; Andrew Barron; Nathalie Bason; Stephen D Bentley; Carol Chillingworth; Tracey Chillingworth; Carol Churcher; Louise Clark; Craig Corton; Ann Cronin; Jon Doggett; Linda Dowd; Theresa Feltwell; Zahra Hance; Barbara Harris; Heidi Hauser; Simon Holroyd; Kay Jagels; Keith D James; Nicola Lennard; Alexandra Line; Rebecca Mayes; Sharon Moule; Karen Mungall; Douglas Ormond; Michael A Quail; Ester Rabbinowitsch; Kim Rutherford; Mandy Sanders; Sarah Sharp; Mark Simmonds; Kim Stevens; Sally Whitehead; Bart G Barrell; Brian G Spratt; Julian Parkhill
Journal: Proc Natl Acad Sci U S A Date: 2004-06-22 Impact factor: 11.205

Multiplex PCR assay for identification of six different Staphylococcus spp. and simultaneous detection of methicillin and mupirocin resistance.

1. Rapid and accurate identification of human-associated staphylococci by use of multiplex PCR.

2. Development of a rapid PCR assay specific for Staphylococcus saprophyticus and application to direct detection from urine samples.

3. Staphylococcus aureus- the predominant pathogen in the neonatal ICU of a tertiary care hospital in amritsar, India.

4. Molecular epidemiology of plasmid-mediated high-level mupirocin resistance in methicillin-resistant Staphylococcus aureus in four Spanish health care settings.

5. Multiplex PCR assay to identify methicillin-resistant Staphylococcus haemolyticus.

6. Comprehensive strategy including prophylactic mupirocin to reduce Staphylococcus aureus colonization and infection in high-risk neonates.

7. Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance.

Review 8. Evolution of community- and healthcare-associated methicillin-resistant Staphylococcus aureus.

9. Multicomponent synthesis of antibacterial dihydropyridin and dihydropyran embelin derivatives.

Review 10. Nasal decolonization of Staphylococcus aureus with mupirocin: strengths, weaknesses and future prospects.

1. Cross-sectional study of Staphyloccus lugdunensis prevalence in cats.

Review 2. DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables.

3. Molecular characterization of clinical isolates from vascular access infection: A single-institution study.