Literature DB >> 2482842

Rapid detection of HIV-1 in clinical samples by co-culture with heat-shocked cells.

M C Re1, G Furlini, M La Placa.   

Abstract

A mild heat-shock (10 min at 44 degrees C), performed just before seeding on healthy PBL used for co-cultivation, allows the detection of viral markers (p24 core antigen and reverse transcriptase) in a very short time. This suggests that heat-shocked PBL can be employed routinely for the rapid detection of HIV-1 in clinical samples.

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Year:  1989        PMID： 2482842     DOI： 10.1016/0166-0934(89)90113-4

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  6 in total

Review 1.  Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Authors:  J H Hughes
Journal:  Clin Microbiol Rev       Date:  1993-04       Impact factor: 26.132

2.  Immunoblotting analysis of IgA and IgM antibody to human immunodeficiency virus type 1 (HIV-1) polypeptides in seropositive infants.

Authors:  M C Re; G Furlini; M Vignoli; G Zauli; P Dallacasa; M Masi; M La Placa
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-01       Impact factor: 3.267

3.  The impaired number of circulating granulocyte/macrophage progenitors (CFU-GM) in human immunodeficiency virus-type 1 infected subjects correlates with an active HIV-1 replication.

Authors:  M C Re; G Zauli; G Furlini; S Ranieri; P Monari; E Ramazzotti; M La Placa
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

4.  Heat shock induces HIV-1 replication in chronically infected promyelocyte cell line OM10.1.

Authors:  K Hashimoto; M Baba; K Gohnai; M Sato; S Shigeta
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

5.  Quantification of human immunodeficiency virus type 1-infected mononuclear cells in peripheral blood of seropositive subjects by newly developed flow cytometry analysis of the product of an in situ PCR assay.

Authors:  M C Re; G Furlini; D Gibellini; M Vignoli; E Ramazzotti; E Lolli; S Ranieri; M La Placa
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948

6.  Cellular heat shock factor 1 positively regulates human immunodeficiency virus-1 gene expression and replication by two distinct pathways.

Authors:  Pratima Rawat; Debashis Mitra
Journal:  Nucleic Acids Res       Date:  2011-04-01       Impact factor: 16.971
  6 in total

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