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Literature DB >> 2482764

Effect of uridine dethiolation in the anticodon triplet of tRNA(Glu) on its association with tRNA(Phe).

C Houssier¹, P Degée, K Nicoghosian, H Grosjean.

Abstract

The effect of U(34) dethiolation on the anticodon-anticodon association between E. coli tRNA(Glu) and yeast tRNA(Phe) has been studied by the temperature jump relaxation technique. An important destabilization upon replacement of the thioketo group of s2U(34) by a keto group, was revealed by a lowering of melting temperature of about 20 degrees C. The measured kinetic parameters indicated that this destabilization effect was originated in an increase of dissociation and a decrease of association rate constants by a factor of 4 to 5. Modifications in both stacking interactions and flexibility in the anticodon loop would be responsible for this effect.

Entities: Chemical Disease Species

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Year: 1988 PMID： 2482764 DOI： 10.1080/07391102.1988.10506468

Source DB: PubMed Journal: J Biomol Struct Dyn ISSN： 0739-1102

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Cited

11 in total

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Effect of uridine dethiolation in the anticodon triplet of tRNA(Glu) on its association with tRNA(Phe).

Review 1. A gripping tale of ribosomal frameshifting: extragenic suppressors of frameshift mutations spotlight P-site realignment.

2. Base modification pattern at the wobble position of Xenopus selenocysteine tRNA(Sec).

3. Natural 2',5'-phosphodiester bonds found at the ligation sites of peach latent mosaic viroid.

4. Synthesis and studies on the effect of 2-thiouridine and 4-thiouridine on sugar conformation and RNA duplex stability.

5. The crystal structure of HIV reverse-transcription primer tRNA(Lys,3) shows a canonical anticodon loop.

6. Surface plasmon resonance kinetic studies of the HIV TAR RNA kissing hairpin complex and its stabilization by 2-thiouridine modification.

7. Single atom modification (O-->S) of tRNA confers ribosome binding.

8. A conserved modified wobble nucleoside (mcm5s2U) in lysyl-tRNA is required for viability in yeast.

9. Unusual anticodon loop structure found in E.coli lysine tRNA.

Review 10. Decoding the genome: a modified view.