Literature DB >> 24821614

Selective detection and quantification of modified DNA with solid-state nanopores.

Autumn T Carlsen1, Osama K Zahid, Jan A Ruzicka, Ethan W Taylor, Adam R Hall.   

Abstract

We demonstrate a solid-state nanopore assay for the unambiguous discrimination and quantification of modified DNA. Individual streptavidin proteins are employed as high-affinity tags for DNA containing a single biotin moiety. We establish that the rate of translocation events corresponds directly to relative concentration of protein-DNA complexes and use the selectivity of our approach to quantify modified oligonucleotides from among a background of unmodified DNA in solution.

Entities:  

Keywords:  DNA; DNA modifications; Solid-state nanopores; proteins; quantification; single-molecule

Mesh：

Substances：

Year:  2014        PMID： 24821614     DOI： 10.1021/nl501340d

Source DB:  PubMed          Journal:  Nano Lett        ISSN： 1530-6984            Impact factor:   11.189


  23 in total

1.  Monovalent Strep-Tactin for strong and site-specific tethering in nanospectroscopy.

Authors:  Fabian Baumann; Magnus S Bauer; Lukas F Milles; Alexander Alexandrovich; Hermann E Gaub; Diana A Pippig
Journal:  Nat Nanotechnol       Date:  2015-10-12       Impact factor: 39.213

2.  Solid-state nanopore localization by controlled breakdown of selectively thinned membranes.

Authors:  Autumn T Carlsen; Kyle Briggs; Adam R Hall; Vincent Tabard-Cossa
Journal:  Nanotechnology       Date:  2017-01-03       Impact factor: 3.874

3.  Smooth DNA transport through a narrowed pore geometry.

Authors:  Spencer Carson; James Wilson; Aleksei Aksimentiev; Meni Wanunu
Journal:  Biophys J       Date:  2014-11-18       Impact factor: 4.033

Review 4.  Nanopore Sensing.

Authors:  Wenqing Shi; Alicia K Friedman; Lane A Baker
Journal:  Anal Chem       Date:  2016-11-18       Impact factor: 6.986

5.  Simple and Efficient Room-Temperature Release of Biotinylated Nucleic Acids from Streptavidin and Its Application to Selective Molecular Detection.

Authors:  Samuel Bearden; Fanny Wang; Adam R Hall
Journal:  Anal Chem       Date:  2019-06-12       Impact factor: 6.986

6.  Modeling and Analysis of Intercalant Effects on Circular DNA Conformation.

Authors:  Eric Krueger; Jiwook Shim; Arman Fathizadeh; Angela Nicole Chang; Basheer Subei; Katie M Yocham; Paul H Davis; Elton Graugnard; Fatemeh Khalili-Araghi; Rashid Bashir; David Estrada; Daniel Fologea
Journal:  ACS Nano       Date:  2016-08-29       Impact factor: 15.881

Review 7.  Microfluidic epigenomic mapping technologies for precision medicine.

Authors:  Chengyu Deng; Lynette B Naler; Chang Lu
Journal:  Lab Chip       Date:  2019-07-24       Impact factor: 6.799

8.  Solid-State Nanopore Analysis of Diverse DNA Base Modifications Using a Modular Enzymatic Labeling Process.

Authors:  Fanny Wang; Osama K Zahid; Brandi E Swain; Derek Parsonage; Thomas Hollis; Scott Harvey; Fred W Perrino; Rahul M Kohli; Ethan W Taylor; Adam R Hall
Journal:  Nano Lett       Date:  2017-10-05       Impact factor: 11.189

9.  Sequence-Specific Recognition of MicroRNAs and Other Short Nucleic Acids with Solid-State Nanopores.

Authors:  Osama K Zahid; Fanny Wang; Jan A Ruzicka; Ethan W Taylor; Adam R Hall
Journal:  Nano Lett       Date:  2016-02-02       Impact factor: 11.189

10.  Direct Detection of Conserved Viral Sequences and Other Nucleic Acid Motifs with Solid-State Nanopores.

Authors:  Komal Sethi; Gabrielle P Dailey; Osama K Zahid; Ethan W Taylor; Jan A Ruzicka; Adam R Hall
Journal:  ACS Nano       Date:  2021-04-29       Impact factor: 15.881
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