Cristina Prat-Vidal1, Carolina Gálvez-Montón2, Verónica Puig-Sanvicens3, Benjamin Sanchez4, Idoia Díaz-Güemes5, Paco Bogónez-Franco4, Isaac Perea-Gil2, Anna Casas-Solà2, Santiago Roura2, Aida Llucià-Valldeperas2, Carolina Soler-Botija2, Francisco M Sánchez-Margallo5, Carlos E Semino3, Ramon Bragos4, Antoni Bayes-Genis6. 1. ICREC (Heart Failure and Cardiac Regeneration) Research Lab, Health Sciences Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain. Electronic address: cprat@igtp.cat. 2. ICREC (Heart Failure and Cardiac Regeneration) Research Lab, Health Sciences Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain. 3. Department of Bioengineering, Institut Químic de Sarrià (IQS), Universitat Ramon Llull, Barcelona, Spain. 4. Electronic and Biomedical Instrumentation Group, Departament d'Enginyeria Electrònica, Universitat Politècnica de Catalunya (UPC), Barcelona, Spain. 5. Jesús Usón Minimally Invasive Surgery Centre, JUMISC, Cáceres, Spain. 6. ICREC (Heart Failure and Cardiac Regeneration) Research Lab, Health Sciences Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain; Department of Medicine, University Autonomous of Barcelona (UAB), Barcelona, Spain. Electronic address: abayesgenis@gmail.com.
Abstract
BACKGROUND/ OBJECTIVES: The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized. METHODS: Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n=8 treated and n=5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections. RESULTS: Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67(+)-GFP(+)-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin(+)-GFP(+)-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation. CONCLUSIONS: With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.
BACKGROUND/ OBJECTIVES: The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized. METHODS: Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n=8 treated and n=5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections. RESULTS: Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67(+)-GFP(+)-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin(+)-GFP(+)-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation. CONCLUSIONS: With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.
Authors: Isaac Perea-Gil; Juan J Uriarte; Cristina Prat-Vidal; Carolina Gálvez-Montón; Santiago Roura; Aida Llucià-Valldeperas; Carolina Soler-Botija; Ramon Farré; Daniel Navajas; Antoni Bayes-Genis Journal: Am J Transl Res Date: 2015-03-15 Impact factor: 4.060
Authors: Antoni Bayes-Genis; Paloma Gastelurrutia; Maria-Luisa Cámara; Albert Teis; Josep Lupón; Cinta Llibre; Elisabet Zamora; Xavier Alomar; Xavier Ruyra; Santiago Roura; Ana Revilla; José Alberto San Román; Carolina Gálvez-Montón Journal: EBioMedicine Date: 2016-04-10 Impact factor: 8.143
Authors: Marta Monguió-Tortajada; Santiago Roura; Carolina Gálvez-Montón; Marcella Franquesa; Antoni Bayes-Genis; Francesc E Borràs Journal: Front Immunol Date: 2017-11-20 Impact factor: 7.561
Authors: Isaac Perea-Gil; Carolina Gálvez-Montón; Cristina Prat-Vidal; Ignasi Jorba; Cristina Segú-Vergés; Santiago Roura; Carolina Soler-Botija; Oriol Iborra-Egea; Elena Revuelta-López; Marco A Fernández; Ramon Farré; Daniel Navajas; Antoni Bayes-Genis Journal: Sci Rep Date: 2018-04-30 Impact factor: 4.379