Literature DB >> 24818929

Transposon mutagenesis.

Hemantha D Kulasekara1.   

Abstract

Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we present a detailed protocol for using Himar1 mariner-based transposon mutagenesis to create mutant libraries in P. aeruginosa.

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Year:  2014        PMID： 24818929     DOI： 10.1007/978-1-4939-0473-0_39

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Conceptual Model of Biofilm Antibiotic Tolerance That Integrates Phenomena of Diffusion, Metabolism, Gene Expression, and Physiology.

Authors:  Philip S Stewart; Ben White; Laura Boegli; Timothy Hamerly; Kerry S Williamson; Michael J Franklin; Brian Bothner; Garth A James; Steve Fisher; Francisco G Vital-Lopez; Anders Wallqvist
Journal:  J Bacteriol       Date:  2019-10-21       Impact factor: 3.490

2.  Regional Control of Chromosome Segregation in Pseudomonas aeruginosa.

Authors:  Valentine Lagage; Frédéric Boccard; Isabelle Vallet-Gely
Journal:  PLoS Genet       Date:  2016-11-07       Impact factor: 5.917

3.  Identification of Drug Resistance Determinants in a Clinical Isolate of Pseudomonas aeruginosa by High-Density Transposon Mutagenesis.

Authors:  Michael S Sonnabend; Kristina Klein; Sina Beier; Angel Angelov; Robert Kluj; Christoph Mayer; Caspar Groß; Kathrin Hofmeister; Antonia Beuttner; Matthias Willmann; Silke Peter; Philipp Oberhettinger; Annika Schmidt; Ingo B Autenrieth; Monika Schütz; Erwin Bohn
Journal:  Antimicrob Agents Chemother       Date:  2020-02-21       Impact factor: 5.191

4.  Identification of genetic determinants of hemolytic activity of Riemerella anatipestifer using random transposon mutagenesis.

Authors:  Bingqing Sun; Yafei Xue; Xiaoli Du; Xiaohua He; Zuocheng Zou; Xiangqiang Tian; Zhonghao Hu; Haoyang Liu; Nazrul Islam; Qinghai Hu
Journal:  Vet Res       Date:  2021-02-12       Impact factor: 3.683

5.  RpoN/Sfa2-dependent activation of the Pseudomonas aeruginosa H2-T6SS and its cognate arsenal of antibacterial toxins.

Authors:  Luke P Allsopp; Alice C Z Collins; Eleanor Hawkins; Thomas E Wood; Alain Filloux
Journal:  Nucleic Acids Res       Date:  2022-01-11       Impact factor: 16.971

6.  RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.

Authors:  Luke P Allsopp; Thomas E Wood; Sophie A Howard; Federica Maggiorelli; Laura M Nolan; Sarah Wettstadt; Alain Filloux
Journal:  Proc Natl Acad Sci U S A       Date:  2017-07-03       Impact factor: 11.205

7.  Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms.

Authors:  Jens Bo Andersen; Louise Dahl Hultqvist; Charlotte Uldahl Jansen; Tim Holm Jakobsen; Martin Nilsson; Morten Rybtke; Jesper Uhd; Blaine Gabriel Fritz; Roland Seifert; Jens Berthelsen; Thomas Eiland Nielsen; Katrine Qvortrup; Michael Givskov; Tim Tolker-Nielsen
Journal:  NPJ Biofilms Microbiomes       Date:  2021-07-09       Impact factor: 7.290
  7 in total

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