| Literature DB >> 24818916 |
Suzanne E Kern1, Alexa Price-Whelan, Dianne K Newman.
Abstract
Nicotinamide adenine dinucleotides are critical redox-active substrates for countless catabolic and anabolic reactions. Ratios of NAD(+) to NADH and NADP(+) to NADPH are therefore considered key indicators of the overall intracellular redox potential and metabolic state. These ratios can be measured in bulk conditions using a highly sensitive enzyme cycling-based colorimetric assay (detection limit at or below 0.05 μM or 1 pmol) following a simple extraction procedure involving solutions of acid and base. Special considerations are necessary to avoid measurement artifacts caused by the presence of endogenous redox-active metabolites, such as phenazines made by diverse Pseudomonas species (see Chapter 25).Entities:
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Year: 2014 PMID： 24818916 DOI： 10.1007/978-1-4939-0473-0_26
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745