Literature DB >> 2481694

CD20 positive human B lymphocytes separated with the magnetic cell sorter (MACS) can be induced to proliferation and antibody secretion in vitro.

H Abts1, M Emmerich, S Miltenyi, A Radbruch, H Tesch.   

Abstract

A method is described for the efficient purification of human B lymphocytes from peripheral blood by magnetic separation. Biotinylated, superparamagnetic particles were coupled to target cells by fluorescein isothiocyanate conjugated avidin and biotinylated monoclonal antibodies directed against cell surface antigens. This combination permitted flow cytometric control of the magnetic separation. Ficoll-Paque-separated peripheral blood mononuclear cells were first eliminated from monocytes by leucine-methyl ester treatment. B cells were enriched to 97% after magnetic depletion of CD3-positive T cells and magnetic enrichment of CD20-positive B cells. The separated B cells could be induced to proliferation and antibody production by various in vitro stimuli.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2481694     DOI： 10.1016/0022-1759(89)90073-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

1.  Differential analysis of human leukocytes by dielectrophoretic field-flow-fractionation.

Authors:  J Yang; Y Huang; X B Wang; F F Becker; P R Gascoyne
Journal:  Biophys J       Date:  2000-05       Impact factor: 4.033

2.  Synthesis of anti-acetylcholine receptor antibodies by CD5- B cells from peripheral blood of myasthenia gravis patients.

Authors:  F Heidenreich; T Jovin
Journal:  J Neurol       Date:  1996-01       Impact factor: 4.849

3.  A simplified method for Ca2+ flux measurement on isolated human B cells that uses flow cytometry.

Authors:  L Gergely; L Cook; V Agnello
Journal:  Clin Diagn Lab Immunol       Date:  1997-01

4.  A distinct wave of human T cell receptor gamma/delta lymphocytes in the early fetal thymus: evidence for controlled gene rearrangement and cytokine production.

Authors:  M S Krangel; H Yssel; C Brocklehurst; H Spits
Journal:  J Exp Med       Date:  1990-09-01       Impact factor: 14.307

5.  Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement.

Authors:  Sandra L Pena Luengas; Gustavo Horacio Marin; Kevin Aviles; Ricardo Cruz Acuña; Gustavo Roque; Felipe Rodríguez Nieto; Francisco Sanchez; Adrián Tarditi; Luis Rivera; Eduardo Mansilla
Journal:  Cancer Biother Radiopharm       Date:  2014-12       Impact factor: 3.099

6.  A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2.

Authors:  T G Diacovo; A R deFougerolles; D F Bainton; T A Springer
Journal:  J Clin Invest       Date:  1994-09       Impact factor: 14.808

Review 7.  Utility of magnetic cell separation as a molecular sperm preparation technique.

Authors:  Tamer M Said; Ashok Agarwal; Maciej Zborowski; Sonja Grunewald; Hans-Juergen Glander; Uwe Paasch
Journal:  J Androl       Date:  2007-12-12

8.  Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function.

Authors:  Mia J Smith; Thomas A Packard; Shannon K O'Neill; Rochelle M Hinman; Marynette Rihanek; Peter A Gottlieb; John C Cambier
Journal:  J Vis Exp       Date:  2017-02-16       Impact factor: 1.355

9.  Long-term in vitro persistence of magnetic properties after magnetic bead-based cell separation of T cells.

Authors:  Aicha Laghmouchi; Conny Hoogstraten; J H Frederik Falkenburg; Inge Jedema
Journal:  Scand J Immunol       Date:  2020-07-17       Impact factor: 3.487

10.  Interactions of human alpha/beta and gamma/delta T lymphocyte subsets in shear flow with E-selectin and P-selectin.

Authors:  T G Diacovo; S J Roth; C T Morita; J P Rosat; M B Brenner; T A Springer
Journal:  J Exp Med       Date:  1996-03-01       Impact factor: 14.307
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.