Literature DB >> 2481674

cDNA molecular cloning of Geotrichum candidum lipase.

Y Shimada1, A Sugihara, Y Tominaga, T Iizumi, S Tsunasawa.   

Abstract

The cDNA clone of Geotrichum candidum (Geo.) lipase was isolated from the Geo. cDNA library by colony hybridization using 32P-labeled oligonucleotides corresponding to a partial amino acid sequence of this enzyme. The nucleotide sequence of the cDNA determined by the dideoxy chain terminating method included some partial amino acid sequences determined by Edman degradation, and the overall amino acid composition deduced from the cDNA coincided with that from amino acid analysis of this protein. The cloned cDNA coded a protein of 554 amino acids and a hydrophobic signal sequence of 19 amino acids. Geo. lipase contained the -Gly-X-Ser-X-Gly- sequence which is believed to form part of the interfacial lipid recognition site.

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Year:  1989        PMID： 2481674     DOI： 10.1093/oxfordjournals.jbchem.a122862

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  14 in total

1.  Does sequence similarity of human choline esterase, Torpedo acetylcholine esterase and Geotrichum candidum lipase reveal the active site serine residue?

Authors:  A R Slabas; J Windust; C M Sidebottom
Journal:  Biochem J       Date:  1990-07-01       Impact factor: 3.857

2.  Identification of Geotrichum candidum at the species and strain level: proposal for a standardized protocol.

Authors:  S Gente; D Sohier; E Coton; C Duhamel; M Gueguen
Journal:  J Ind Microbiol Biotechnol       Date:  2006-07-20       Impact factor: 3.346

3.  Cholinesterase-like domains in enzymes and structural proteins: functional and evolutionary relationships and identification of a catalytically essential aspartic acid.

Authors:  E Krejci; N Duval; A Chatonnet; P Vincens; J Massoulié
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-01       Impact factor: 11.205

4.  An Aspergillus awamori acetylesterase: purification of the enzyme, and cloning and sequencing of the gene.

Authors:  T Koseki; S Furuse; K Iwano; H Sakai; H Matsuzawa
Journal:  Biochem J       Date:  1997-09-01       Impact factor: 3.857

Review 5.  Relationship between sequence conservation and three-dimensional structure in a large family of esterases, lipases, and related proteins.

Authors:  M Cygler; J D Schrag; J L Sussman; M Harel; I Silman; M K Gentry; B P Doctor
Journal:  Protein Sci       Date:  1993-03       Impact factor: 6.725

6.  A prespore-cell-inducing factor in Dictyostelium discoideum: its purification and characterization.

Authors:  M Nakagawa; A A Oohata; H Tojo; S Fujii
Journal:  Biochem J       Date:  1999-10-01       Impact factor: 3.857

7.  Purification and specificity of lipases fromGeotrichum candidum.

Authors:  M W Baillargeon
Journal:  Lipids       Date:  1990-12       Impact factor: 1.880

8.  Cloning and expression of Aspergillus tamarii FS132 lipase gene in Pichia pastoris.

Authors:  Bihong Shi; Liqing Zeng; Haolei Song; Qiaoqin Shi; Songgang Wu
Journal:  Int J Mol Sci       Date:  2010-06-03       Impact factor: 5.923

9.  Purification and properties of an Arthrobacter oxydans P52 carbamate hydrolase specific for the herbicide phenmedipham and nucleotide sequence of the corresponding gene.

Authors:  H D Pohlenz; W Boidol; I Schüttke; W R Streber
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

10.  Cloning of the Pseudomonas glumae lipase gene and determination of the active site residues.

Authors:  L G Frenken; M R Egmond; A M Batenburg; J W Bos; C Visser; C T Verrips
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792
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