Literature DB >> 24816671

qPCR for quantification of transgene expression and determination of transgene copy number.

Stephen J Fletcher1.   

Abstract

Quantitative real-time PCR (qPCR) is a mature technology that can be used to accurately quantify the number of copies of a target nucleic acid in a sample. Here, we describe a method for using this technology to determine the copy number of a transgene stably integrated into a plant's genome and to ascertain the level of transgene expression.

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Year:  2014        PMID： 24816671     DOI： 10.1007/978-1-4939-0446-4_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Wheat drought-responsive WXPL transcription factors regulate cuticle biosynthesis genes.

Authors:  Huihui Bi; Sukanya Luang; Yuan Li; Natalia Bazanova; Nikolai Borisjuk; Maria Hrmova; Sergiy Lopato
Journal:  Plant Mol Biol       Date:  2017-02-04       Impact factor: 4.076

2.  Engineering sulfur storage in maize seed proteins without apparent yield loss.

Authors:  Jose Planta; Xiaoli Xiang; Thomas Leustek; Joachim Messing
Journal:  Proc Natl Acad Sci U S A       Date:  2017-10-09       Impact factor: 11.205

3.  The wheat TabZIP2 transcription factor is activated by the nutrient starvation-responsive SnRK3/CIPK protein kinase.

Authors:  Sukanya Luang; Pradeep Sornaraj; Natalia Bazanova; Wei Jia; Omid Eini; Syed Sarfraz Hussain; Nataliya Kovalchuk; Pradeep K Agarwal; Maria Hrmova; Sergiy Lopato
Journal:  Plant Mol Biol       Date:  2018-03-21       Impact factor: 4.076

4.  Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis.

Authors:  Huihui Bi; Sukanya Luang; Yuan Li; Natalia Bazanova; Sarah Morran; Zhihong Song; M Ann Perera; Maria Hrmova; Nikolai Borisjuk; Sergiy Lopato
Journal:  J Exp Bot       Date:  2016-08-03       Impact factor: 6.992

5.  Constitutive overexpression of the TaNF-YB4 gene in transgenic wheat significantly improves grain yield.

Authors:  Dinesh Yadav; Yuri Shavrukov; Natalia Bazanova; Larissa Chirkova; Nikolai Borisjuk; Nataliya Kovalchuk; Ainur Ismagul; Boris Parent; Peter Langridge; Maria Hrmova; Sergiy Lopato
Journal:  J Exp Bot       Date:  2015-07-27       Impact factor: 6.992

6.  Molecular identification of the wheat male fertility gene Ms1 and its prospects for hybrid breeding.

Authors:  Elise J Tucker; Ute Baumann; Allan Kouidri; Radoslaw Suchecki; Mathieu Baes; Melissa Garcia; Takashi Okada; Chongmei Dong; Yongzhong Wu; Ajay Sandhu; Manjit Singh; Peter Langridge; Petra Wolters; Marc C Albertsen; A Mark Cigan; Ryan Whitford
Journal:  Nat Commun       Date:  2017-10-11       Impact factor: 14.919
  6 in total

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