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Literature DB >> 2481633

Protein heterogeneity of Francisella tularensis: detection of proteins with antigenic determinants.

J Stulik¹, J Cerna, H Kovarová, A Macela.

Abstract

The autolyzates of three different strains of Francisella tularensis 15L, 130 and SCHU were tested for their immunogenic potential and protein heterogeneity. The autolyzates induce the production of specific antibodies, the delayed type of hypersensitivity, and some degree of protection against European virulent strain 130. This material (as antigen) was especially suitable for ELISA. When the autolyzates were subjected to SDS gradient PAGE, a variety of polypeptides were distinguished. The composition of polypeptides from all three strains on SDS-PAGE was almost identical. After the detection of antigenic determinants by Western blotting more than twenty bands appeared. The visualization with polyclonal antisera against live laboratory strain 15L and against autolyzates 15L, 130 and SCHU revealed differences in the composition of the antigenic determinants of these strains.

Entities: Chemical Disease Species

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Year: 1989 PMID： 2481633 DOI： 10.1007/bf02814474

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

17 in total

1. Studies on Bacterium tularense antigens. I. The isolation, purification, and biologic activity of antigen preparations from Bacterium tularense.

Authors: R A ORMSBEE; J F BELL; C L LARSON
Journal: J Immunol Date: 1955-05 Impact factor: 5.422

2. The development of resistance in mice immunized with soluble antigen derived from Bacterium tularense.

Authors: C L LARSON; J F BELL; C R OWEN
Journal: J Immunol Date: 1954-10 Impact factor: 5.422

3. Interleukin 2 and gamma interferon production, interleukin 2 receptor expression, and DNA synthesis induced by tularemia antigen in vitro after natural infection or vaccination.

Authors: R Karttunen; G Andersson; H P Ekre; K Juutinen; H M Surcel; H Syrjälä; E Herva
Journal: J Clin Microbiol Date: 1987-06 Impact factor: 5.948

4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

Protein heterogeneity of Francisella tularensis: detection of proteins with antigenic determinants.

1. Studies on Bacterium tularense antigens. I. The isolation, purification, and biologic activity of antigen preparations from Bacterium tularense.

2. The development of resistance in mice immunized with soluble antigen derived from Bacterium tularense.

3. Interleukin 2 and gamma interferon production, interleukin 2 receptor expression, and DNA synthesis induced by tularemia antigen in vitro after natural infection or vaccination.

4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Enzyme-linked immunosorbent assay for immunological diagnosis of human tularemia.

7. Antibody response of swine to outer membrane components of Haemophilus pleuropneumoniae during infection.

8. Cell-mediated and humoral immunity induced by a live Francisella tularensis vaccine.

9. Antigens of Pasteurella tularensis: preparative procedures.

10. Immunospecific T-lymphocyte stimulation by membrane proteins from Francisella tularensis.

1. The production of oxygen metabolites and their possible regulatory role in the course of tularemia infection.