Luiz Guilherme Martins Maia1, Angela Valéria Farias Alves2, Talita Santos Bastos3, Lucas Sandes Moromizato4, Isabel Bezerra Lima-Verde5, Maria Amália Gonzaga Ribeiro6, Luiz Gonzaga Gandini Júnior7, Ricardo Luiz Cavalcanti de Albuquerque-Júnior8. 1. School of Dentistry, University Tiradentes (UNIT), Rua Terêncio Sampaio, 309, Grageru, Aracaju 49025700, Sergipe, Brazil. Electronic address: orthomaia2003@yahoo.com.br. 2. Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: angela.dilela@yahoo.com.br. 3. Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: talibiomed12@gmail.com. 4. Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: lucasmoromizato@hotmail.com. 5. Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: isabel_limaverde@yahoo.com.br. 6. Department of Dentistry, Federal University of Sergipe (UFS), Avenida Cláudio Batista, 54, Sanatório, Aracaju 49000-000, Sergipe, Brazil. Electronic address: endoribeiro@yahoo.com.br. 7. Department of Dentistry, State University Júlio de Mesquita (UNESP), Rua Humaitá, Centro, 1680, Araraquara 14801-903, São Paulo, Brazil. Electronic address: lgandini@foar.unesp.br. 8. School of Dentistry, University Tiradentes (UNIT), Rua Terêncio Sampaio, 309, Grageru, Aracaju 49025700, Sergipe, Brazil; Laboratory of Morphology and Structural Biology, Science and Technology Institute (ITP), Avenida Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju 49032-490, Sergipe, Brazil. Electronic address: ricardo.patologia@uol.com.br.
Abstract
OBJECTIVE: The purpose of this research was to evaluate the histological changes of the periodontal ligament and alveolar bone during dental movement in diabetic rats subjected to low level laser therapy (LLLT). METHODS: The movement of the upper molar was performed in 60 male Wistar rats divided into four groups (n=15): CTR (control), DBT (diabetic), CTR/LT (irradiated control) and DBT/LT (irradiated diabetic). Diabetes was induced with alloxan (150 mg/kg, i.p.). LLLT was applied with GaAlAs laser at 780 nm (35 J/cm(2)). After 7, 13 and 19 days, the periodontal ligament and alveolar bone were histologically analyzed. RESULTS: The mean of osteoblasts (p<0.01) and blood vessels (p<0.05) were significantly decreased in DBT compared with CTR at 7 days, whereas the mean of osteoclasts was lower at 7 (p<0.001) and 13 days (p<0.05). In DBT/LT, only the mean of osteoclasts was lower than in CTR (p<0.05) at 7 days, but no difference was observed at 13 and 19 days (p>0.05). The collagenization of the periodontal ligament was impaired in DBT, whereas DBT/LLT showed density/disposition of the collagen fibers similar to those observed in CTR. CONCLUSIONS: LLLT improved the periodontal ligament and alveolar bone remodeling activity in diabetic rats during dental movement.
OBJECTIVE: The purpose of this research was to evaluate the histological changes of the periodontal ligament and alveolar bone during dental movement in diabeticrats subjected to low level laser therapy (LLLT). METHODS: The movement of the upper molar was performed in 60 male Wistar rats divided into four groups (n=15): CTR (control), DBT (diabetic), CTR/LT (irradiated control) and DBT/LT (irradiated diabetic). Diabetes was induced with alloxan (150 mg/kg, i.p.). LLLT was applied with GaAlAs laser at 780 nm (35 J/cm(2)). After 7, 13 and 19 days, the periodontal ligament and alveolar bone were histologically analyzed. RESULTS: The mean of osteoblasts (p<0.01) and blood vessels (p<0.05) were significantly decreased in DBT compared with CTR at 7 days, whereas the mean of osteoclasts was lower at 7 (p<0.001) and 13 days (p<0.05). In DBT/LT, only the mean of osteoclasts was lower than in CTR (p<0.05) at 7 days, but no difference was observed at 13 and 19 days (p>0.05). The collagenization of the periodontal ligament was impaired in DBT, whereas DBT/LLT showed density/disposition of the collagen fibers similar to those observed in CTR. CONCLUSIONS: LLLT improved the periodontal ligament and alveolar bone remodeling activity in diabeticrats during dental movement.
Authors: Mehmet Ali Karabel; Mehmet Doğru; Arzum Doğru; Mehmet İrfan Karadede; Mehmet Cudi Tuncer Journal: Acta Cir Bras Date: 2021-01-20 Impact factor: 1.388