| Literature DB >> 24814706 |
Prabhat Pratap Singh Tomar1, Kumar Nikhil1, Anamika Singh1, Purushotham Selvakumar1, Partha Roy1, Ashwani Kumar Sharma2.
Abstract
The plant 2S albumins exhibit a spectrum of biotechnologically exploitable functions. Among them, pumpkin 2S albumin has been shown to possess RNase and cell-free translational inhibitory activities. The present study investigated the anticancer, DNase and antifungal activities of pumpkin 2S albumin. The protein exhibited a strong anticancer activity toward breast cancer (MCF-7), ovarian teratocarcinoma (PA-1), prostate cancer (PC-3 and DU-145) and hepatocellular carcinoma (HepG2) cell lines. Acridine orange staining and DNA fragmentation studies indicated that cytotoxic effect of pumpkin 2S albumin is mediated through induction of apoptosis. Pumpkin 2S albumin showed DNase activity against both supercoiled and linear DNA and exerted antifungal activity against Fusarium oxysporum. Secondary structure analysis by CD showed that protein is highly stable up to 90°C and retains its alpha helical structure. These results demonstrated that pumpkin 2S albumin is a multifunctional protein with host of potential biotechnology applications.Entities:
Keywords: Anticancer activity; Antifungal activity; CD studies; Deoxyribonuclease activity; Pumpkin 2S albumin
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Year: 2014 PMID： 24814706 DOI： 10.1016/j.bbrc.2014.04.158
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575