Literature DB >> 24814478

A rapid qPCR method for genetic sex identification of Salmo salar and Salmo trutta including simultaneous elucidation of interspecies hybrid paternity by high-resolution melt analysis.

M B Anglès d'Auriac1, H A Urke, T Kristensen.   

Abstract

This study presents an improved duplex quantitative polymerase chain reaction (qPCR) method using the master sex-determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar × Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes.
© 2014 The Fisheries Society of the British Isles.

Entities:  

Keywords:  aquaculture; fish ecology; molecular diagnostic; molecular sexing; sdY gene; species substitution

Mesh：

Substances：

Year:  2014        PMID： 24814478     DOI： 10.1111/jfb.12401

Source DB:  PubMed          Journal:  J Fish Biol        ISSN： 0022-1112            Impact factor:   2.051


  5 in total

1.  A qPCR-duplex assay for sex determination in ancient DNA.

Authors:  Anna Poma; Patrizia Cesare; Antonella Bonfigli; Anna Rita Volpe; Sabrina Colafarina; Giulia Vecchiotti; Alfonso Forgione; Osvaldo Zarivi
Journal:  PLoS One       Date:  2022-06-10       Impact factor: 3.752

2.  COMplementary Primer ASymmetric PCR (COMPAS-PCR) Applied to the Identification of Salmo salar, Salmo trutta and Their Hybrids.

Authors:  Marc B Anglès d'Auriac
Journal:  PLoS One       Date:  2016-10-26       Impact factor: 3.240

3.  High Rate of Deformed Larvae among Gynogenetic Brown Trout (Salmo trutta m. fario) Doubled Haploids.

Authors:  Krzysztof Jagiełło; Tomasz Zalewski; Stefan Dobosz; Oliwia Michalik; Konrad Ocalewicz
Journal:  Biomed Res Int       Date:  2017-04-09       Impact factor: 3.411

4.  Efficient coralline algal psbA mini barcoding and High Resolution Melt (HRM) analysis using a simple custom DNA preparation.

Authors:  Marc B Anglès d'Auriac; Line Le Gall; Viviana Peña; Jason M Hall-Spencer; Robert S Steneck; Stein Fredriksen; Janne Gitmark; Hartvig Christie; Vivian Husa; Ellen Sofie Grefsrud; Eli Rinde
Journal:  Sci Rep       Date:  2019-01-24       Impact factor: 4.379

5.  Mapping of Adaptive Traits Enabled by a High-Density Linkage Map for Lake Trout.

Authors:  Seth R Smith; Stephen J Amish; Louis Bernatchez; Jeremy Le Luyer; Chris C Wilson; Olivia Boeberitz; Gordon Luikart; Kim T Scribner
Journal:  G3 (Bethesda)       Date:  2020-06-01       Impact factor: 3.154
  5 in total

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