Yuanyuan Ran1, Ran Wang1, Murtaza Hasan1, Qiutian Jia1, Bo Tang1, Shuangquan Shan1, Yulin Deng2, Hong Qing3. 1. School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China. 2. School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: deng@bit.edu.cn. 3. School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: hqing@bit.edu.cn.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Dragon׳s blood, a traditional Chinese herb, has been used to "panacea of blood activating" and its major biological activity appears to be from phenolic compounds. In this study, our research aims to examine the effects of Dragon׳s blood (DB) and its extracts (DBE) on radiation-induced myelosuppressive mice. MATERIALS AND METHODS: Adult BALB/C mice were exposed to the whole body irradiation with 4 Gy (60)Co γ-rays. DB and DBE were respectively administered orally for 5 constitutive days prior to irradiation treatment. The radioprotective effects and relevant mechanisms of DB and DBE in radiation-induced bone marrow injury were investigated by ex vivo examination. RESULTS: We found that the administration of DB and DBE significantly increased the numbers of peripheral blood cells and colony forming unit of bone marrow-derived stem/progenitor cells. Interestingly, compared with the irradiation group, the administration of DB and DBE significantly decreased the levels of the inflammatory cytokines such as IL-6, TNF-α and IFN-γ and oxidative stress injury such as SOD, CAT, GSH, MDA in serum of mice. Furthermore, DBE markedly improved the morphology of bone marrow histopathology. CONCLUSIONS: Our data suggest that DB and DBE effectively attenuate radiation-induced damage in bone marrow, which is likely associated with the anti-oxidative and anti-inflammatory properties of DB and DBE.
ETHNOPHARMACOLOGICAL RELEVANCE: Dragon׳s blood, a traditional Chinese herb, has been used to "panacea of blood activating" and its major biological activity appears to be from phenolic compounds. In this study, our research aims to examine the effects of Dragon׳s blood (DB) and its extracts (DBE) on radiation-induced myelosuppressive mice. MATERIALS AND METHODS: Adult BALB/C mice were exposed to the whole body irradiation with 4 Gy (60)Co γ-rays. DB and DBE were respectively administered orally for 5 constitutive days prior to irradiation treatment. The radioprotective effects and relevant mechanisms of DB and DBE in radiation-induced bone marrow injury were investigated by ex vivo examination. RESULTS: We found that the administration of DB and DBE significantly increased the numbers of peripheral blood cells and colony forming unit of bone marrow-derived stem/progenitor cells. Interestingly, compared with the irradiation group, the administration of DB and DBE significantly decreased the levels of the inflammatory cytokines such as IL-6, TNF-α and IFN-γ and oxidative stress injury such as SOD, CAT, GSH, MDA in serum of mice. Furthermore, DBE markedly improved the morphology of bone marrow histopathology. CONCLUSIONS: Our data suggest that DB and DBE effectively attenuate radiation-induced damage in bone marrow, which is likely associated with the anti-oxidative and anti-inflammatory properties of DB and DBE.
Authors: Tran Thi Van Anh; Alilou Mostafa; Zhigang Rao; Simona Pace; Stefan Schwaiger; Christian Kretzer; Veronika Temml; Carsten Giesel; Paul M Jordan; Rossella Bilancia; Christina Weinigel; Silke Rummler; Birgit Waltenberger; Tran Hung; Antonietta Rossi; Hermann Stuppner; Oliver Werz; Andreas Koeberle Journal: Acta Pharm Sin B Date: 2021-04-20 Impact factor: 11.413