Xuanyi Li1, Xi Shi2, Yuehua Qiao3, Kailin Xu4, Lingyu Zeng4, Caiji Wang1, Zhou Xu5, Haichen Niu2. 1. Department of Otolaryngology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China. 2. Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou 221006, China. 3. Department of Otolaryngology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China; Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou 221006, China. Electronic address: oto8558@163.com. 4. Laboratory of Transplantation Immunity of Xuzhou Medical College, Xuzhou 221006, China. 5. School of Basic Education Science of Xuzhou Medical College, Xuzhou 221006, China.
Abstract
OBJECTIVE: Congenital cytomegalovirus (CMV) infection is the most common infectious cause of sensorineural hearing loss in children. This study aims to investigate the pathogenesis CMV-induced hearing loss from the view of integrity of blood-labyrinth-barrier (BLB). METHODS: Newborn BALB/c mice were randomly divided into three groups (n=22, respectively): CMV group, control group and normal group. The CMV group and control group were intracerebrally injected with equal volume (15 μl) of murine CMV (MCMV; 10(4)IU/0.1 ml) and PBS, respectively, and normal group did not receive any treatment. After three weeks, auditory-evoked brainstem response was assessed, and permeability of BLB was evaluated by Evans blue method. Means between groups were compared using t-test. RESULTS: We observed that mice injected with MCMV had a hearing loss and it was connected with the permeability changes of BLB. Besides, using hematoxylin-eosin staining, we noticed hyperaemia in stria vascularis and spiral ligament and bleeding in scala vestibule and scala tympani in CMV group. CONCLUSION: All these data indicated the possible association between CMV-induced hearing loss and BLB dysfunction with the characteristics of inflammation. Our data provide a possible path to investigate the mechanism of CMV-induced hearing damage.
OBJECTIVE:Congenital cytomegalovirus (CMV) infection is the most common infectious cause of sensorineural hearing loss in children. This study aims to investigate the pathogenesis CMV-induced hearing loss from the view of integrity of blood-labyrinth-barrier (BLB). METHODS: Newborn BALB/c mice were randomly divided into three groups (n=22, respectively): CMV group, control group and normal group. The CMV group and control group were intracerebrally injected with equal volume (15 μl) of murine CMV (MCMV; 10(4)IU/0.1 ml) and PBS, respectively, and normal group did not receive any treatment. After three weeks, auditory-evoked brainstem response was assessed, and permeability of BLB was evaluated by Evans blue method. Means between groups were compared using t-test. RESULTS: We observed that mice injected with MCMV had a hearing loss and it was connected with the permeability changes of BLB. Besides, using hematoxylin-eosin staining, we noticed hyperaemia in stria vascularis and spiral ligament and bleeding in scala vestibule and scala tympani in CMV group. CONCLUSION: All these data indicated the possible association between CMV-induced hearing loss and BLB dysfunction with the characteristics of inflammation. Our data provide a possible path to investigate the mechanism of CMV-induced hearing damage.
Authors: Hao Chang; Ravindra S Telang; Sreevalsan Sreebhavan; Malcolm Tingle; Peter R Thorne; Srdjan M Vlajkovic Journal: Biomed Res Int Date: 2017-01-18 Impact factor: 3.411