T Ebert1, L M Hopf2, U Wurst2, A Bachmann3, S Kralisch2, U Lössner2, M Platz2, J Kratzsch4, J U Stolzenburg5, A Dietel5, O Grisk6, J Beige7, M Anders8, I Bast8, N Klöting9, M Blüher3, M Stumvoll3, M Fasshauer2. 1. University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany. Electronic address: thomas.ebert@medizin.uni-leipzig.de. 2. University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany; Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany. 3. University of Leipzig, Department of Endocrinology and Nephrology, 04103 Leipzig, Germany. 4. University of Leipzig, Institute of Laboratory Medicine, 04103 Leipzig, Germany. 5. University of Leipzig, Department of Urology, 04103 Leipzig, Germany. 6. University of Greifswald, Department of Physiology, 17495 Greifswald, Karlsburg, Germany. 7. Hospital St. Georg, Division of Nephrology and KfH Renal Unit, 04129 Leipzig, Germany. 8. Outpatient Nephrology Care Unit, 04107 and 04178 Leipzig, Germany. 9. Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
Abstract
BACKGROUND AND AIMS: The adipokine adipocyte fatty acid binding protein (AFABP) is positively associated with the development of the metabolic syndrome, diabetes mellitus, and cardiovascular disease. We hypothesized that AFABP also increases with deteriorating renal function. METHODS AND RESULTS: Serum AFABP levels were quantified by enzyme linked immunosorbent assay in 532 patients with chronic kidney disease (CKD) covering the whole spectrum of estimated glomerular filtration rate (eGFR) categories from G1 to G5 (study population 1). Furthermore, AFABP was measured in 32 patients before and within 30 h after elective unilateral nephrectomy, a model of acute kidney dysfunction (AKD) (study population 2). Moreover, circulating AFABP was investigated in rats undergoing bilateral nephrectomy (BNE) as compared to sham-operated animals. Median serum AFABP levels adjusted for age, gender, and body mass index significantly increased with increasing eGFR category (G1: 22.0 μg/l; G2: 34.6 μg/l; G3: 56.7 μg/l; G4: 95.2 μg/l; and G5: 173.9 μg/l). Furthermore, renal dysfunction remained positively associated with AFABP in multivariate analysis in this cohort. In patients undergoing unilateral nephrectomy, AFABP increased significantly after surgery (42.1 μg/l) as compared to pre-surgical values (29.3 μg/l). Furthermore, relative changes of post-to-pre-surgical AFABP levels were independently associated with relative changes of post-to-pre-surgical creatinine concentrations. After BNE in rats, AFABP increased significantly as compared to sham-operated animals. CONCLUSIONS: We show that AFABP is significantly elevated in CKD and AKD patients. Furthermore, measures of renal function are associated with circulating AFABP. Moreover, animal experiments indicate that AFABP levels strongly depend on renal function.
BACKGROUND AND AIMS: The adipokine adipocyte fatty acid binding protein (AFABP) is positively associated with the development of the metabolic syndrome, diabetes mellitus, and cardiovascular disease. We hypothesized that AFABP also increases with deteriorating renal function. METHODS AND RESULTS: Serum AFABP levels were quantified by enzyme linked immunosorbent assay in 532 patients with chronic kidney disease (CKD) covering the whole spectrum of estimated glomerular filtration rate (eGFR) categories from G1 to G5 (study population 1). Furthermore, AFABP was measured in 32 patients before and within 30 h after elective unilateral nephrectomy, a model of acute kidney dysfunction (AKD) (study population 2). Moreover, circulating AFABP was investigated in rats undergoing bilateral nephrectomy (BNE) as compared to sham-operated animals. Median serum AFABP levels adjusted for age, gender, and body mass index significantly increased with increasing eGFR category (G1: 22.0 μg/l; G2: 34.6 μg/l; G3: 56.7 μg/l; G4: 95.2 μg/l; and G5: 173.9 μg/l). Furthermore, renal dysfunction remained positively associated with AFABP in multivariate analysis in this cohort. In patients undergoing unilateral nephrectomy, AFABP increased significantly after surgery (42.1 μg/l) as compared to pre-surgical values (29.3 μg/l). Furthermore, relative changes of post-to-pre-surgical AFABP levels were independently associated with relative changes of post-to-pre-surgical creatinine concentrations. After BNE in rats, AFABP increased significantly as compared to sham-operated animals. CONCLUSIONS: We show that AFABP is significantly elevated in CKD and AKD patients. Furthermore, measures of renal function are associated with circulating AFABP. Moreover, animal experiments indicate that AFABP levels strongly depend on renal function.
Authors: Thomas Ebert; Susan Kralisch; Nora Klöting; Annett Hoffmann; Matthias Blüher; Ming-Zhi Zhang; Raymond C Harris; Michael Stumvoll; Mathias Fasshauer Journal: Kidney Int Date: 2015-11 Impact factor: 10.612
Authors: Chi Ho Lee; Chloe Y Y Cheung; Yu Cho Woo; David T W Lui; Michele M A Yuen; Carol H Y Fong; Wing Sun Chow; Amin Xu; Karen S L Lam Journal: Diabetologia Date: 2018-09-28 Impact factor: 10.122
Authors: Rongshuang Huang; Min Shi; Fan Guo; Yuying Feng; Yanhuan Feng; Jing Liu; Lingzhi Li; Yan Liang; Jin Xiang; Song Lei; Liang Ma; Ping Fu Journal: Front Pharmacol Date: 2018-08-08 Impact factor: 5.810