PURPOSE: To evaluate the morphological and histological changes induced by PGA scaffold seeded with autologous adipose or muscle derived stem cells implanted on rabbit bladder wall. MATERIAL AND METHODS: Adipose derived stem cells (ADSCs) were obtained from the inguinal fat of eight rabbits and muscle derived stem cells (MDSCs) from the anterior tibial muscle of other eight rabbits. After culture and isolation, the cells were stained with Vybrant Red CM DiI and then implanted at third passage. Two PGA scaffolds were implanted on the bladder submucosa of each animal. On the right bladder side was implanted unseeded PGA scaffold while on the left side was implanted ADSCs or skeletal MDSCs seeded PGA scaffold. ADSCs were implanted in eight animals and MDSC in other eight animals. The animals were sacrificed at four and eight weeks. Histological evaluation was performed with Hematoxylin and Eosin, Masson's Trichrome and smooth muscle α-actin. RESULTS: We observed a mild inflammatory response in all the three groups. Seeded scaffolds induced higher lymphocytes and lower polimorphonuclear migration than controls. Fibrosis was more pronounced in the control groups. Smooth muscle α-actin was positive only in ADSC and MDSC seeded scaffolds. At four and eight weeks ADCSs and skeletal MDSCs labeled cells were found at the implant sites. CONCLUSIONS: The implantation of PGA scaffolds seeded with ADSC and MDSC induced less fibrosis than control and smooth muscle regeneration.
PURPOSE: To evaluate the morphological and histological changes induced by PGA scaffold seeded with autologous adipose or muscle derived stem cells implanted on rabbit bladder wall. MATERIAL AND METHODS: Adipose derived stem cells (ADSCs) were obtained from the inguinal fat of eight rabbits and muscle derived stem cells (MDSCs) from the anterior tibial muscle of other eight rabbits. After culture and isolation, the cells were stained with Vybrant Red CM DiI and then implanted at third passage. Two PGA scaffolds were implanted on the bladder submucosa of each animal. On the right bladder side was implanted unseeded PGA scaffold while on the left side was implanted ADSCs or skeletal MDSCs seeded PGA scaffold. ADSCs were implanted in eight animals and MDSC in other eight animals. The animals were sacrificed at four and eight weeks. Histological evaluation was performed with Hematoxylin and Eosin, Masson's Trichrome and smooth muscle α-actin. RESULTS: We observed a mild inflammatory response in all the three groups. Seeded scaffolds induced higher lymphocytes and lower polimorphonuclear migration than controls. Fibrosis was more pronounced in the control groups. Smooth muscle α-actin was positive only in ADSC and MDSC seeded scaffolds. At four and eight weeks ADCSs and skeletal MDSCs labeled cells were found at the implant sites. CONCLUSIONS: The implantation of PGA scaffolds seeded with ADSC and MDSC induced less fibrosis than control and smooth muscle regeneration.
Authors: Dongdeuk Kwon; Yongtae Kim; Ryan Pruchnic; Ron Jankowski; Irma Usiene; Fernando de Miguel; Johnny Huard; Michael B Chancellor Journal: Urology Date: 2006-08 Impact factor: 2.649
Authors: Gregory S Jack; Fernando G Almeida; Rong Zhang; Zeni C Alfonso; Patricia A Zuk; Larissa V Rodríguez Journal: J Urol Date: 2005-11 Impact factor: 7.450
Authors: Larissa V Rodríguez; Zeni Alfonso; Rong Zhang; Joanne Leung; Benjamin Wu; Louis J Ignarro Journal: Proc Natl Acad Sci U S A Date: 2006-07-31 Impact factor: 11.205
Authors: Kshitiz Raj Shrestha; Seung Hwan Jeon; Ae Ryang Jung; In Gul Kim; Ga Eun Kim; Yong Hyun Park; Soo Hyun Kim; Ji Youl Lee Journal: Tissue Eng Regen Med Date: 2019-03-22 Impact factor: 4.169
Authors: Jun Nyung Lee; So Young Chun; Hyo-Jung Lee; Yu-Jin Jang; Seock Hwan Choi; Dae Hwan Kim; Se Heang Oh; Phil Hyun Song; Jin Ho Lee; Jong Kun Kim; Tae Gyun Kwon Journal: J Korean Med Sci Date: 2015-11-30 Impact factor: 2.153
Authors: Paulina García-Estrada; Miguel A García-Bon; Edgar J López-Naranjo; Dulce N Basaldúa-Pérez; Arturo Santos; Jose Navarro-Partida Journal: Pharmaceutics Date: 2021-05-12 Impact factor: 6.321