Histological studies on the effects of pineal 5-methoxyindoles on the reproductive organs of the male golden hamster.

The effects of late-afternoon injections of melatonin (MEL), 5-methoxytryptamine (MTN), 5-methoxytryptophol (MTOL), and 5-methoxyindole-3-acetic acid (MIAA) on testicular and seminal vesicular histology in the golden hamster were examined. MEL, MTN, and MTOL injections caused a reduction in the diameters of seminiferous tubules and an inhibition of spermatogenesis. Testicular regression ranged from a decrease in the abundance of late spermatids and mature spermatozoa in some animals to an almost complete loss of spermatogenesis in others. Sertoli cells were more resistant to the treatment than other cellular components of the seminiferous tubules. Leydig cells were reduced in size, showed a great reduction in cytoplasm, and possessed shrunken and angular nuclei. The epithelial cells of seminal vesicles were reduced in size and became cuboidal or low columnar. Some secretory cells possessed pyknotic nuclei and had minimal secretory activity. MTN and MTOL appeared to be more potent than MEL in inducing the aforementioned changes, whereas MIAA failed to exert similar effects.