Literature DB >> 24808658

Molecular detection of Sarcocystis species in slaughtered sheep by PCR-RFLP from south-western of Iran.

Hossein Hamidinejat1, Hossein Moetamedi2, Alireza Alborzi1, Abbas Hatami3.   

Abstract

Sarcocystis spp. are the cyst forming protozoan parasites that prevalent in livestock all around the world. In the presented work, we examined 40 macroscopic and 40 microscopic sarcocysts from Khouzestan and Lorestan provinces, south-western Iran, utilizing PCR-RFLP based on amplification of 18S rRNA gene. Using AvaI, HindII, TaqI and EcoRI restriction enzymes the results represented Sarcocystis gigantea in both macroscopic and microscopic cysts. This result supports the importance of molecular investigations on characterization of Sarcocystis species when we aimed to assess the reliable control and preventive programs.

Entities:  

Keywords:  18S rRNA; Iran; PCR–RFLP; Sarcocystis spp.; Sheep

Year:  2013        PMID： 24808658      PMCID： PMC4000366          DOI： 10.1007/s12639-012-0231-z

Source DB:  PubMed          Journal:  J Parasit Dis        ISSN： 0971-7196


  23 in total

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