Literature DB >> 2480783

Establishment of strongly neutralizing monoclonal antibody to human interleukin-6 and its epitope analysis.

N Ida1, S Sakurai, T Hosaka, K Hosoi, T Kunitomo, T Shimazu, T Maruyama, Y Matsuura, M Kohase.   

Abstract

Three monoclonal antibodies against human interleukin-6 were established and characterized. One antibody was shown to strongly neutralize both the Ig-inducing and hybridoma/plasmacytoma growth activity of interleukin-6. The results of its epitope analysis using protease treated interleukin-6 and immobilized antibody indicated that this neutralizing antibody binds to a peptide corresponding to Leu151-Lys171 of interleukin-6 molecule. Further analysis using synthetic peptides showed that a shorter peptide corresponding to Ala153-Thr162 can also inhibit the binding of the antibody to interleukin-6. These results suggest that this carboxyl-terminal region plays a crucial role in interleukin-6 functions.

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Year:  1989        PMID： 2480783     DOI： 10.1016/s0006-291x(89)80027-0

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  8 in total

Review 1.  Interleukin-6: structure-function relationships.

Authors:  R J Simpson; A Hammacher; D K Smith; J M Matthews; L D Ward
Journal:  Protein Sci       Date:  1997-05       Impact factor: 6.725

2.  Structure-function relationships of interleukin-3. An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine interleukin-3.

Authors:  K Kaushansky; S G Shoemaker; V C Broudy; N L Lin; J V Matous; E M Alderman; J D Aghajanian; P J Szklut; R E VanDyke; M K Pearce
Journal:  J Clin Invest       Date:  1992-11       Impact factor: 14.808

3.  Characterization of cytokine production in infectious mononucleosis studied at a single-cell level in tonsil and peripheral blood.

Authors:  J Andersson; U Andersson
Journal:  Clin Exp Immunol       Date:  1993-04       Impact factor: 4.330

4.  Interleukin 6 production by peripheral blood mononuclear cells in patients with chronic hepatitis B virus infection and primary biliary cirrhosis.

Authors:  S Kakumu; T Shinagawa; T Ishikawa; K Yoshioka; T Wakita; N Ida
Journal:  Gastroenterol Jpn       Date:  1993-02

5.  Structure-function analysis of human IL-6: identification of two distinct regions that are important for receptor binding.

Authors:  A Hammacher; L D Ward; J Weinstock; H Treutlein; K Yasukawa; R J Simpson
Journal:  Protein Sci       Date:  1994-12       Impact factor: 6.725

6.  Cells transfected with human interleukin 6 cDNA acquire binding sites for the hepatitis B virus envelope protein.

Authors:  A R Neurath; N Strick; Y Y Li
Journal:  J Exp Med       Date:  1992-12-01       Impact factor: 14.307

7.  Norovirus (NoV) specific protective immune responses induced by recombinant P dimer vaccine are enhanced by the mucosal adjuvant FlaB.

Authors:  Vivek Verma; Wenzhi Tan; Sao Puth; Kyoung-Oh Cho; Shee Eun Lee; Joon Haeng Rhee
Journal:  J Transl Med       Date:  2016-05-17       Impact factor: 5.531

8.  Production of multiple growth factors by a newly established human thyroid carcinoma cell line.

Authors:  K Tohyama; Y Yoshida; K Ohashi; E Sano; H Kobayashi; K Endo; M Naruto; T Nakamura
Journal:  Jpn J Cancer Res       Date:  1992-02
  8 in total

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