Keke Jia1, Chuanbao Zhang2, Xianzhang Huang3, Lanlan Wang4, Xiaoke Hao5, Runqing Mu6, Baishen Pan7, Jie Zhang1, Wenxiang Chen2, Ning Xu3, Guixing Li4, Yueyun Ma5, Ming Ma6, Wei Guo7, Hong Shang6. 1. Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China. 2. National Center for Clinical Laboratories, Beijing Hospital, Beijing, China. 3. Department of Laboratory Science, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong, China. 4. Department of Laboratory Medicine, West China Hospital, Sichuan University, Sichuan, China. 5. Department of Clinical Laboratory Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China. 6. Department of Laboratory Medicine, the First Affiliated Hospital of China Medical University, Shenyang, China. 7. Department of Clinical Laboratory Medicine, Zhongshan Hospital of Fudan University, Shanghai, China.
Abstract
BACKGROUND: Currently there are no reference intervals (RIs) of sodium (Na), potassium (K), and chlorine (Cl) on Chinese population. Two kinds of ion-selective electrode (ISE) methods were commonly used to determine K, Na, and Cl levels in China, the difference between these two methods needs to be evaluated. METHODS: A total of 4,524 healthy participants (1,916 males and 2,608 females) between 20-79 years old from six cities in China were selected by strict criteria. Serum K, Na, and CL were tested on Roche Modular analyzers in six assigned laboratories. According to EP-9A2, using Roche Modular analyzer (indirect ISE) as comparative method, Olympus AU 5400 analyzer (indirect ISE) and Johnson&Johnson Fusion 5.1 analyzer (direct ISE) were evaluated. RESULTS: In Chinese population, the RIs for K, Na, and CL are 3.6-5.2, 136-146, and 99-110 mmol/l, respectively. Compared to the Roche indirect ISE method, Johnson direct ISE method showed a positive bias; and Olympus indirect ISE method just showed a very slight bias. CONCLUSION: The RIs of K, Na, and Cl of Han Chinese healthy adult population were found to be smaller than those provided by the manufacturer. By a criteria of biological variations for CV, the differences of Na and K between Roche analyzer and Johnson analyzer were not acceptable for clinical application, while the differences of Na, K, and Cl between Roche and Olympus analyzers were acceptable for clinical application.
BACKGROUND: Currently there are no reference intervals (RIs) of sodium (Na), potassium (K), and chlorine (Cl) on Chinese population. Two kinds of ion-selective electrode (ISE) methods were commonly used to determine K, Na, and Cl levels in China, the difference between these two methods needs to be evaluated. METHODS: A total of 4,524 healthy participants (1,916 males and 2,608 females) between 20-79 years old from six cities in China were selected by strict criteria. Serum K, Na, and CL were tested on Roche Modular analyzers in six assigned laboratories. According to EP-9A2, using Roche Modular analyzer (indirect ISE) as comparative method, Olympus AU 5400 analyzer (indirect ISE) and Johnson&Johnson Fusion 5.1 analyzer (direct ISE) were evaluated. RESULTS: In Chinese population, the RIs for K, Na, and CL are 3.6-5.2, 136-146, and 99-110 mmol/l, respectively. Compared to the Roche indirect ISE method, Johnson direct ISE method showed a positive bias; and Olympus indirect ISE method just showed a very slight bias. CONCLUSION: The RIs of K, Na, and Cl of Han Chinese healthy adult population were found to be smaller than those provided by the manufacturer. By a criteria of biological variations for CV, the differences of Na and K between Roche analyzer and Johnson analyzer were not acceptable for clinical application, while the differences of Na, K, and Cl between Roche and Olympus analyzers were acceptable for clinical application.
Authors: Emma K Southcott; Jennifer L Kerrigan; Julia M Potter; Richard D Telford; Paul Waring; Graham J Reynolds; Antony R A Lafferty; Peter E Hickman Journal: Clin Chim Acta Date: 2010-06-22 Impact factor: 3.786
Authors: Kiyoshi Ichihara; Yoshihisa Itoh; Christopher W K Lam; Priscilla M K Poon; Jeong-Ho Kim; Hiroshi Kyono; Naning Chandrawening; Dewi Muliaty Journal: Clin Chem Date: 2007-12-18 Impact factor: 8.327