Lukasz Gilowski1, Rafał Wiench2, Iwona Płocica2, Tadeusz Faustyn Krzemiński3. 1. Chair and Department of Pharmacology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Jordana 38, 41-808 Zabrze, Poland; Private Dental Practice, Gliwice, Poland. Electronic address: aplg@wp.pl. 2. Chair and Department of Conservative Dentistry with Endodontics, School of Medicine with the Division of Dentistry, Medical University of Silesia, Bytom, Poland. 3. Chair and Department of Pharmacology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Jordana 38, 41-808 Zabrze, Poland.
Abstract
OBJECTIVE: Regulation of interleukin-1β (IL-1β) is a critical element of immune responses in health and disease. Additional research is required to determine the levels of interleukin-1 receptor antagonist (IL-1ra) sufficient to inhibit inteleukine-1-induced responses in periodontium. The aim of this study was to compare the levels of IL-1β, IL-1ra in gingival crevicular fluid samples obtained from periodontitis and healthy patients. DESIGN OF THE STUDY: The periodontitis group consisted of 50 patients (F-31, M-19) with chronic periodontitis. The control group consisted of 30 periodontally healthy subjects (F-19, M-11). Oral hygiene index-simplified, gingival index, pocket depth, clinical attachment level were measured. The concentrations of interleukins in gingival crevicular fluid were measured by the ELISA technique. RESULTS: The total amounts of interleukin-1β and IL-1ra were significantly higher in periodontitis group than in the control group (P<0.05). The levels of each cytokine were reported also as moles. In the control group there were 800-times more moles/sample of IL-1ra than IL-1β. In periodontitis group nearly 300-times more moles/sample of IL-1ra was obtained. The significant positive Spearman's rank correlations was identified between both concentrations (R=0.39; P<0.05) and total amounts (R=0.31; P<0.05) of cytokines in the periodontitis group. CONCLUSION: The increased secretion of IL-1ra in periodontitis is not adequate to "curb" the release of IL-1β.
OBJECTIVE: Regulation of interleukin-1β (IL-1β) is a critical element of immune responses in health and disease. Additional research is required to determine the levels of interleukin-1 receptor antagonist (IL-1ra) sufficient to inhibit inteleukine-1-induced responses in periodontium. The aim of this study was to compare the levels of IL-1β, IL-1ra in gingival crevicular fluid samples obtained from periodontitis and healthy patients. DESIGN OF THE STUDY: The periodontitis group consisted of 50 patients (F-31, M-19) with chronic periodontitis. The control group consisted of 30 periodontally healthy subjects (F-19, M-11). Oral hygiene index-simplified, gingival index, pocket depth, clinical attachment level were measured. The concentrations of interleukins in gingival crevicular fluid were measured by the ELISA technique. RESULTS: The total amounts of interleukin-1β and IL-1ra were significantly higher in periodontitis group than in the control group (P<0.05). The levels of each cytokine were reported also as moles. In the control group there were 800-times more moles/sample of IL-1ra than IL-1β. In periodontitis group nearly 300-times more moles/sample of IL-1ra was obtained. The significant positive Spearman's rank correlations was identified between both concentrations (R=0.39; P<0.05) and total amounts (R=0.31; P<0.05) of cytokines in the periodontitis group. CONCLUSION: The increased secretion of IL-1ra in periodontitis is not adequate to "curb" the release of IL-1β.
Authors: Erica N Recker; Kim A Brogden; Gustavo Avila-Ortiz; Carol L Fischer; Keyla Pagan-Rivera; Deborah V Dawson; Katherine M Smith; Satheesh Elangovan Journal: Arch Oral Biol Date: 2015-07-14 Impact factor: 2.633
Authors: Muhammad M Majeed; Imtiaz Ahmed; Talat Roome; Yasser Alali; Khulud A Al-Aali; Naseer Ahmed; Zohra Saleem; Abdulkareem A Alhumaidan; Waqas A Farooqui; Saeeda Ahmed; Fahim Vohra; Tariq Abduljabbar Journal: Int J Environ Res Public Health Date: 2022-03-02 Impact factor: 4.614