Jingjie Li1, Zhihong Yang2, Zheng Li1, Lijuan Gu1, Yunbo Wang1, Changkeun Sung3. 1. Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea. 2. College of Life Science, Huzhou University, Huzhou, Zhejiang 31300, PR China. 3. Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: kchsung@cnu.ac.kr.
Abstract
OBJECTIVE: Insulin-like growth factor 1 (IGF-1) increases the growth of cultured hair follicles and plays a role in regulating hair migration during the development of hair follicles in transgenic mice. However, the exogenous effect of IGF-1 on hair growth in wild-type mice has not been reported. In the present study, we examined whether IGF-1 was an important regulator of hair follicle growth in wide-type mice in vivo. DESIGN: C57BL/6 mice were injected with different concentrations of IGF-1 on dorsal skin. The treated tissues were analyzed by immunoassay methods for TGF-β1 and BrdU. RESULTS: Local injection of IGF-1 increased hair follicle number and prolonged the growing phase during the transition from anagen to telogen. Meanwhile, immunology analyses revealed that IGF-1 also stimulated the proliferation of follicle cells in anagen of the matrix and down regulated TGF-β1 expression in hair follicles. CONCLUSIONS: These observations suggest that IGF-1 is an effective stimulator of hair follicle development in wide-type mice in vivo and may be a promising drug candidate for baldness therapy.
OBJECTIVE:Insulin-like growth factor 1 (IGF-1) increases the growth of cultured hair follicles and plays a role in regulating hair migration during the development of hair follicles in transgenic mice. However, the exogenous effect of IGF-1 on hair growth in wild-type mice has not been reported. In the present study, we examined whether IGF-1 was an important regulator of hair follicle growth in wide-type mice in vivo. DESIGN: C57BL/6 mice were injected with different concentrations of IGF-1 on dorsal skin. The treated tissues were analyzed by immunoassay methods for TGF-β1 and BrdU. RESULTS: Local injection of IGF-1 increased hair follicle number and prolonged the growing phase during the transition from anagen to telogen. Meanwhile, immunology analyses revealed that IGF-1 also stimulated the proliferation of follicle cells in anagen of the matrix and down regulated TGF-β1 expression in hair follicles. CONCLUSIONS: These observations suggest that IGF-1 is an effective stimulator of hair follicle development in wide-type mice in vivo and may be a promising drug candidate for baldness therapy.
Authors: Parvathy Thampi; Rashmi Dubey; Rachael Lowney; Emma N Adam; Sarah Janse; Constance L Wood; James N MacLeod Journal: Cartilage Date: 2019-04-25 Impact factor: 3.117