Literature DB >> 2479577

Point mutations in conserved amino acid residues within the C-terminal domain of HIV-1 reverse transcriptase specifically repress RNase H function.

O Schatz1, F V Cromme, F Grüninger-Leitch, S F Le Grice.   

Abstract

Two single site substitutions (E478----Q and H539----F) were introduced into the C-terminal RNase H domain of HIV-1 reverse transcriptase. These mutant proteins were expressed in Escherichia coli and purified by Ni2+-nitrilotriacetic acid affinity chromatography. Both enzymes are clearly defective in RNase H function, but exhibit wild type reverse transcriptase activity.

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Year:  1989        PMID： 2479577     DOI： 10.1016/0014-5793(89)81559-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  57 in total

1.  Defects in Moloney murine leukemia virus replication caused by a reverse transcriptase mutation modeled on the structure of Escherichia coli RNase H.

Authors:  A Telesnitsky; S W Blain; S P Goff
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

2.  Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1.

Authors:  P L Boyer; A L Ferris; S H Hughes
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

3.  Characterization of the double stranded RNA dependent RNase activity associated with recombinant reverse transcriptases.

Authors:  H Ben-Artzi; E Zeelon; S F Le-Grice; M Gorecki; A Panet
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

4.  Comparative analysis of immune responses induced by vaccination with SIV antigens by recombinant Ad5 vector or plasmid DNA in rhesus macaques.

Authors:  Lauren A Hirao; Ling Wu; Abhishek Satishchandran; Amir S Khan; Ruxandra Draghia-Akli; Adam C Finnefrock; Andrew J Bett; Michael R Betts; Danilo R Casimiro; Niranjan Y Sardesai; J Joseph Kim; John W Shiver; David B Weiner
Journal:  Mol Ther       Date:  2010-06-15       Impact factor: 11.454

5.  Nucleotide docking: prediction of reactant state complexes for ribonuclease enzymes.

Authors:  Brigitta Elsässer; Gregor Fels
Journal:  J Mol Model       Date:  2010-12-01       Impact factor: 1.810

6.  Mechanism of HIV reverse transcriptase inhibition by zinc: formation of a highly stable enzyme-(primer-template) complex with profoundly diminished catalytic activity.

Authors:  Katherine J Fenstermacher; Jeffrey J DeStefano
Journal:  J Biol Chem       Date:  2011-09-26       Impact factor: 5.157

7.  Mechanism for nucleoside analog-mediated abrogation of HIV-1 replication: balance between RNase H activity and nucleotide excision.

Authors:  Galina N Nikolenko; Sarah Palmer; Frank Maldarelli; John W Mellors; John M Coffin; Vinay K Pathak
Journal:  Proc Natl Acad Sci U S A       Date:  2005-01-31       Impact factor: 11.205

8.  Tighter binding of HIV reverse transcriptase to RNA-DNA versus DNA-DNA results mostly from interactions in the polymerase domain and requires just a small stretch of RNA-DNA.

Authors:  William P Bohlayer; Jeffrey J DeStefano
Journal:  Biochemistry       Date:  2006-06-20       Impact factor: 3.162

9.  Purification and characterization of an active human immunodeficiency virus type 1 RNase H domain.

Authors:  J S Smith; M J Roth
Journal:  J Virol       Date:  1993-07       Impact factor: 5.103

10.  Hydroxyl radical footprint analysis of human immunodeficiency virus reverse transcriptase-template.primer complexes.

Authors:  W Metzger; T Hermann; O Schatz; S F Le Grice; H Heumann
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205
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