| Literature DB >> 24794442 |
Justin Voog1, Sharsti L Sandall2, Gary R Hime3, Luís Pedro F Resende4, Mariano Loza-Coll5, Aaron Aslanian6, John R Yates7, Tony Hunter6, Margaret T Fuller8, D Leanne Jones9.
Abstract
Stem cells reside within specialized microenvironments, or niches, that control many aspects of stem cell behavior. Somatic hub cells in the Drosophila testis regulate the behavior of cyst stem cells (CySCs) and germline stem cells (GSCs) and are a primary component of the testis stem cell niche. The shutoff (shof) mutation, characterized by premature loss of GSCs and CySCs, was mapped to a locus encoding the evolutionarily conserved transcription factor Escargot (Esg). Hub cells depleted of Esg acquire CySC characteristics and differentiate as cyst cells, resulting in complete loss of hub cells and eventually CySCs and GSCs, similar to the shof mutant phenotype. We identified Esg-interacting proteins and demonstrate an interaction between Esg and the corepressor C-terminal binding protein (CtBP), which was also required for maintenance of hub cell fate. Our results indicate that niche cells can acquire stem cell properties upon removal of a single transcription factor in vivo.Entities:
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Year: 2014 PMID： 24794442 PMCID： PMC4128242 DOI： 10.1016/j.celrep.2014.04.025
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423