Najiba Fekih-Mrissa1, Meriem Mrad2, Anis Riahi3, Aicha Sayeh2, Jamel Zaouali3, Nasreddine Gritli4, Ridha Mrissa3. 1. Hôpital Militaire Principal d'Instruction de Tunis, Service d'Hématologie, Laboratoire de Biologie Moléculaire, 1008 Montfleury, Tunis, Tunisie; Académie Militaire Fondouk Jédid, 8012 Nabeul, Tunisie. Electronic address: fnajiba@yahoo.fr. 2. Hôpital Militaire Principal d'Instruction de Tunis, Service d'Hématologie, Laboratoire de Biologie Moléculaire, 1008 Montfleury, Tunis, Tunisie; Université de Tunis El Manar, Faculté des Sciences de Tunis, 2092, El Manar, Tunisie. 3. Hôpital Militaire Principal d'Instruction de Tunis, Service de Neurologie, 1008 Montfleury, Tunis, Tunisie; Université de Tunis El Manar, Faculté de Médecine de Tunis, 1007 Tunis, Tunisie. 4. Hôpital Militaire Principal d'Instruction de Tunis, Service d'Hématologie, Laboratoire de Biologie Moléculaire, 1008 Montfleury, Tunis, Tunisie; Université de Monastir, Faculté de Pharmacie, 5000 Monastir, Tunisie.
Abstract
UNLABELLED: Human leukocyte antigen (HLA) alleles have been implicated in many autoimmune diseases. The aim of this study is to assess whether HLA-DR/DQ alleles confer susceptibility to Guillain-Barré syndrome (GBS) in a Tunisian population. METHODS: The HLA-DR/DQ genotyping was performed using polymerase chain reaction sequence-specific primers (PCR-SSP) in 38 patients with GBS and 100 healthy Tunisian control subjects. RESULTS: GBS in Tunisian patients was found to be associated with the following alleles with these relative patient versus control frequencies (pc denotes Bonferroni corrected probability values): DRB1*13 (23.68% vs. 9.0%; pc=0.01), followed by DRB1*14 (22.36% vs.5.5%; pc<10(-3)). Two haplotypes, DRB1*14/DQB1*05 and DRB1*13/DQB1*03, were found to be associated with susceptibility to GBS. However DRB1*07/DQB1*02 and DRB1*03/DQB1*02 haplotypes were more frequently observed in controls than in patients (11.5% vs.7.9%; pc=0.007 and 23% vs. 5.26%; pc<10(-3) respectively). These haplotypes seem to confer protection against the disease. CONCLUSION: Our data demonstrated a new GBS predisposition associated with HLA-DRB1*14 and DRB1*13. Theses alleles could be predisposing genetic factors for GBS in the Tunisian population.
UNLABELLED: Human leukocyte antigen (HLA) alleles have been implicated in many autoimmune diseases. The aim of this study is to assess whether HLA-DR/DQ alleles confer susceptibility to Guillain-Barré syndrome (GBS) in a Tunisian population. METHODS: The HLA-DR/DQ genotyping was performed using polymerase chain reaction sequence-specific primers (PCR-SSP) in 38 patients with GBS and 100 healthy Tunisian control subjects. RESULTS: GBS in Tunisian patients was found to be associated with the following alleles with these relative patient versus control frequencies (pc denotes Bonferroni corrected probability values): DRB1*13 (23.68% vs. 9.0%; pc=0.01), followed by DRB1*14 (22.36% vs.5.5%; pc<10(-3)). Two haplotypes, DRB1*14/DQB1*05 and DRB1*13/DQB1*03, were found to be associated with susceptibility to GBS. However DRB1*07/DQB1*02 and DRB1*03/DQB1*02 haplotypes were more frequently observed in controls than in patients (11.5% vs.7.9%; pc=0.007 and 23% vs. 5.26%; pc<10(-3) respectively). These haplotypes seem to confer protection against the disease. CONCLUSION: Our data demonstrated a new GBS predisposition associated with HLA-DRB1*14 and DRB1*13. Theses alleles could be predisposing genetic factors for GBS in the Tunisian population.
Authors: L Schirmer; V Worthington; U Solloch; V Loleit; V Grummel; N Lakdawala; D Grant; R Wassmuth; A H Schmidt; F Gebhardt; T F M Andlauer; J Sauter; A Berthele; M P Lunn; Bernhard Hemmer Journal: J Neurol Date: 2016-08-02 Impact factor: 4.849
Authors: Patrick S L Kwan; Catherine Xavier; Monica Santovenia; Janet Pruckler; Steven Stroika; Kevin Joyce; Tracie Gardner; Patricia I Fields; Joe McLaughlin; Robert V Tauxe; Collette Fitzgerald Journal: Appl Environ Microbiol Date: 2014-08 Impact factor: 4.792
Authors: Zaki N Hasan; Haider H Zalzala; Hyam R Mohammedsalih; Batool M Mahdi; Laheeb A Abid; Zena N Shakir; Maithem J Fadhel Journal: Neurosciences (Riyadh) Date: 2014-10 Impact factor: 0.906