Highly effective hot spots for SERS signatures of live fibroblasts.

Pre-formed silver-boron nanoparticles of 22 nm form pearl-like necklace nanostructures with interparticle junctions of less than 10 nm length in the matrix of polyethylene glycol (8000 Da). The silver necklace nanostructure is stable at 37 °C or 70 °C and also inside a live cell medium. A polyethylene glycol matrix with a shorter chain length (1000 Da) does not protect the nanoparticles against attraction, and random aggregates are formed. Silver necklace nanostructures exhibit strong Raman enhancement by more than ∼10(9) which is much higher than for silver-citrate or random silver-boron aggregates. The polymeric matrix of 8000 Da contributes strongly to the electromagnetic field enhancement and removes the chemical contribution to the surface Raman scattering increase. The stable interparticle junctions act as local hot spots for strong Raman scattering signals collected from live fibroblasts and allow systematic in situ studies.