CONTEXT: Aberrant hypermethylation of promoter region of tumor suppressor genes could be used as cancer biomarkers. OBJECTIVE: To test methylation status of ZAR1 and SFRP4 promoter regions as potentials biomarkers for diagnosis of preneoplastic and neoplastic lesions of cervix. MATERIALS AND METHODS: Cytobrush samples were evaluated by Methylation specific PCR (MSP) and quantitative MSP (qMSP). RESULTS: ZAR1 and SFRP4 methylation frequency increased as the grade of lesion increased and the differences between normal and cervical cancer (CC) are statistically significant (p < 0.0001). qMSP showed higher ZAR1 and SFRP4 methylation levels in cancer than normal epithelia (p < 0.001) and preneoplastics lesions (p < 0.01). DISCUSSION: qMSP quantify methylation levels and have high sensitivity and specificity. CONCLUSION: ZAR1 and SFRP4 qMSP could be used as potential biomarker for CC diagnosis.
CONTEXT: Aberrant hypermethylation of promoter region of tumor suppressor genes could be used as cancer biomarkers. OBJECTIVE: To test methylation status of ZAR1 and SFRP4 promoter regions as potentials biomarkers for diagnosis of preneoplastic and neoplastic lesions of cervix. MATERIALS AND METHODS: Cytobrush samples were evaluated by Methylation specific PCR (MSP) and quantitative MSP (qMSP). RESULTS:ZAR1 and SFRP4 methylation frequency increased as the grade of lesion increased and the differences between normal and cervical cancer (CC) are statistically significant (p < 0.0001). qMSP showed higher ZAR1 and SFRP4 methylation levels in cancer than normal epithelia (p < 0.001) and preneoplastics lesions (p < 0.01). DISCUSSION: qMSP quantify methylation levels and have high sensitivity and specificity. CONCLUSION:ZAR1 and SFRP4 qMSP could be used as potential biomarker for CC diagnosis.
Authors: Verena Deutschmeyer; Janina Breuer; Sara K Walesch; Anna M Sokol; Johannes Graumann; Marek Bartkuhn; Thomas Boettger; Oliver Rossbach; Antje M Richter Journal: Clin Epigenetics Date: 2019-12-04 Impact factor: 6.551
Authors: Kurt Buchegger; Carmen Ili; Ismael Riquelme; Pablo Letelier; Alejandro H Corvalán; Priscilla Brebi; Tim Hui-Ming Huang; Juan Carlos Roa Journal: Biol Res Date: 2016-01-22 Impact factor: 5.612