Alejandra de-la-Torre1, Alexander W Pfaff2, Michael E Grigg3, Odile Villard2, Ermanno Candolfi2, Jorge E Gomez-Marin4. 1. Universidad del Rosario, Escuela de Medicina y Ciencias de la Salud, Departamento de Inmunología, Bogotá, Colombia. 2. Institut de Parasitologie et Pathologie Tropicale, Fédération de Médecine Translationelle de Strasbourg, Université de Strasbourg, France. 3. Laboratory of Parasitic Diseases, National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIAID), Bethesda, MD, USA. 4. GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindío, Avenida Bolivar 12N, Armenia, Colombia. Electronic address: gepamol2@uniquindio.edu.co.
Abstract
PURPOSE: To determine the cytokine levels in aqueous humor (AH) of Colombian patients with active ocular toxoplasmosis (OT), and to correlate them with their clinical characteristics. METHODS: 27 Cytokines/chemokines were assayed in 15 AH samples (nine patients with diagnosis of OT biologically-confirmed and six controls that underwent cataract surgery). Correlations were assessed between cytokine/chemokine levels, type of inflammatory response (Th1, Th2, Th17, Treg), and clinical characteristics. RESULTS: Th2 predominant response was related to more severe clinical features. The presence of VEGF and IL-5 was related to higher number of recurrences. Growth factors (VEGF, FGF, PDGF-β), were related to higher number of lesions. Patients infected by type-I/III strains had a particular intraocular cytokine-pattern. CONCLUSIONS: Th2 response was related to more severe clinical characteristics in patients infected by Type I/III strains. IL-5 and VEGF were associated with recurrences. We correlate for the first time, specific cytokine-patterns with clinical characteristics and with the infecting Toxoplasma strain.
PURPOSE: To determine the cytokine levels in aqueous humor (AH) of Colombian patients with active ocular toxoplasmosis (OT), and to correlate them with their clinical characteristics. METHODS: 27 Cytokines/chemokines were assayed in 15 AH samples (nine patients with diagnosis of OT biologically-confirmed and six controls that underwent cataract surgery). Correlations were assessed between cytokine/chemokine levels, type of inflammatory response (Th1, Th2, Th17, Treg), and clinical characteristics. RESULTS: Th2 predominant response was related to more severe clinical features. The presence of VEGF and IL-5 was related to higher number of recurrences. Growth factors (VEGF, FGF, PDGF-β), were related to higher number of lesions. Patients infected by type-I/III strains had a particular intraocular cytokine-pattern. CONCLUSIONS: Th2 response was related to more severe clinical characteristics in patients infected by Type I/III strains. IL-5 and VEGF were associated with recurrences. We correlate for the first time, specific cytokine-patterns with clinical characteristics and with the infecting Toxoplasma strain.
Authors: C A Naranjo-Galvis; A de-la-Torre; L E Mantilla-Muriel; L Beltrán-Angarita; X Elcoroaristizabal-Martín; R McLeod; N Alliey-Rodriguez; I J Begeman; C López de Mesa; J E Gómez-Marín; J C Sepúlveda-Arias Journal: Infect Immun Date: 2018-03-22 Impact factor: 3.441
Authors: Cristina S Meira; Vera L Pereira-Chioccola; José E Vidal; Cinara C Brandão de Mattos; Gabriela Motoie; Thais A Costa-Silva; Ricardo Gava; Fábio B Frederico; Luiz C de Mattos Journal: Front Microbiol Date: 2014-10-13 Impact factor: 5.640
Authors: Alejandra de-la-Torre; Juanita Valdés-Camacho; Clara López de Mesa; Andrés Uauy-Nazal; Juan David Zuluaga; Lina María Ramírez-Páez; Felipe Durán; Elizabeth Torres-Morales; Jessica Triviño; Mateo Murillo; Alba Cristina Peñaranda; Juan Carlos Sepúlveda-Arias; Jorge Enrique Gómez-Marín Journal: BMC Infect Dis Date: 2019-01-25 Impact factor: 3.090