| Literature DB >> 24786471 |
Li Jiang1, Dongxu He2, Dantong Yang1, Zhen Chen1, Qiongxi Pan1, Aiqin Mao1, Yanfei Cai1, Xiyuan Li1, Hui Xing1, Mei Shi1, Yun Chen1, Iain C Bruce1, Teng Wang3, Linfang Jin3, Xiaowei Qi3, Dong Hua3, Jian Jin4, Xin Ma5.
Abstract
To investigate the role of microRNAs in the development of chemoresistance and related epithelial-mesenchymal transition (EMT), we examined the effect of miR-489 in adriamycin (ADM)-resistant human breast cancer cells (MCF-7/ADM). MiR-489 was significantly suppressed in MCF-7/ADM cells compared with chemosensitive parental control MCF-7/WT cells. Forced-expression of miR-489 reversed chemoresistance. Furthermore, Smad3 was identified as the target of miR-489 and is highly expressed in MCF-7/ADM cells. Forced expression of miR-489 both inhibited Smad3 expression and Smad3 related EMT properties. Finally, the interactions between Smad3, miR-489 and EMT were confirmed in chemoresistant tumor xenografts and clinical samples, indicating their potential implication for treatment of chemoresistance.Entities:
Keywords: Breast cancer; Chemoresistance; Epithelial mesenchymal transition; microRNAs
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Year: 2014 PMID： 24786471 DOI： 10.1016/j.febslet.2014.04.024
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124