Louis Mubangizi1, Fatuma Namusoke1, Twaha Mutyaba2. 1. Department of Obstetrics and Gynecology, Makerere University, Kampala, Uganda. 2. Department of Obstetrics and Gynecology, Makerere University, Kampala, Uganda. Electronic address: tmutyaba@yahoo.com.
Abstract
OBJECTIVE: To determine prevalent aerobic cervical bacteria and sensitivity to commonly used antibiotics in patients with advanced cervical cancer before and after 4 weeks of external beam radiotherapy (EBRT). METHODS: Cervical swabs were collected prior to the initial radiation dose and after 4 weeks of radiotherapy at Mulago Hospital. Aerobic culture was performed on blood agar, chocolate agar, and MacConkey agar, and incubated at 35-37 ° for 24-48 hours. Isolates were identified using colonial morphology, Gram staining, and biochemical analysis. Sensitivity testing was performed via Kirby-Bauer disk diffusion and dilution. Differences in the proportions of bacteria isolated before and after radiotherapy were compared. Paired t test was used to obtain differences in sensitivity before and after radiotherapy. RESULTS: Normal flora increased significantly after EBRT (P=0.02). There was no significant change in overall proportion of positive cultures. Sensitivity to commonly used antibiotics improved (P=0.05) and resistance significantly decreased (P=0.005). Significant differences were seen mainly with ciprofloxacin, ceftriaxone, and gentamicin. CONCLUSION: Four weeks of EBRT did not sterilize the cervix but resulted in an increase in normal flora. Radiotherapy appeared to reduce resistance to commonly used antibiotics. Sensitivity to chloramphenicol was higher than for the more commonly used antibiotics.
OBJECTIVE: To determine prevalent aerobic cervical bacteria and sensitivity to commonly used antibiotics in patients with advanced cervical cancer before and after 4 weeks of external beam radiotherapy (EBRT). METHODS: Cervical swabs were collected prior to the initial radiation dose and after 4 weeks of radiotherapy at Mulago Hospital. Aerobic culture was performed on blood agar, chocolateagar, and MacConkey agar, and incubated at 35-37 ° for 24-48 hours. Isolates were identified using colonial morphology, Gram staining, and biochemical analysis. Sensitivity testing was performed via Kirby-Bauer disk diffusion and dilution. Differences in the proportions of bacteria isolated before and after radiotherapy were compared. Paired t test was used to obtain differences in sensitivity before and after radiotherapy. RESULTS: Normal flora increased significantly after EBRT (P=0.02). There was no significant change in overall proportion of positive cultures. Sensitivity to commonly used antibiotics improved (P=0.05) and resistance significantly decreased (P=0.005). Significant differences were seen mainly with ciprofloxacin, ceftriaxone, and gentamicin. CONCLUSION: Four weeks of EBRT did not sterilize the cervix but resulted in an increase in normal flora. Radiotherapy appeared to reduce resistance to commonly used antibiotics. Sensitivity to chloramphenicol was higher than for the more commonly used antibiotics.
Authors: Brynn A Hollingsworth; David R Cassatt; Andrea L DiCarlo; Carmen I Rios; Merriline M Satyamitra; Thomas A Winters; Lanyn P Taliaferro Journal: Front Pharmacol Date: 2021-05-18 Impact factor: 5.810
Authors: Natalia Zeber-Lubecka; Maria Kulecka; Bogusław Lindner; Ryszard Krynicki; Agnieszka Paziewska; Andrzej Nowakowski; Mariusz Bidzinski; Jerzy Ostrowski Journal: Front Oncol Date: 2022-09-28 Impact factor: 5.738