Rajdeep Pavaskar1, Paul Chalakkal2, Ramesh Krishnan3, Saritha Sirikonda4, Madhu Vasepalli5, Pammi Venkataramana6. 1. Lecturer, Department of Coservative Dentistry and Endodontics, Goa Dental College and Hospital , Bambolim, Goa, India . 2. Lecturer, Department of Pedodontics and Preventive Dentistry, Goa Dental College and Hospital , Bambolim, Goa, India . 3. Professor, Department of Pedodontics and Preventive Dentistry, VMSDC , Salem, Tamil Nadu, India . 4. Senior Lecturer, Department of Pedodontics and Preventive Dentistry, Meghna Institute of Dental Sciences , Post-Mallaram (Varni road), Nizamabad, Andhra Pradesh,India . 5. Professor and HOD, Department of Pedodontics and Preventive Dentistry, KLR'S Lenora Institute of Dental Sciences , Rajanagaram, Rajahmundry, East Godavari, Andhra Pradesh,India . 6. Professor and HOD, Department of Oral Medicine and Radiology, KLR'S Lenora Institute of Dental Sciences , Rajanagaram, Rajahmundry, East Godavari, Andhra Pradesh,India .
Abstract
PURPOSE: This study evaluated the efficacy of 2% chlorhexidine (CX), calcium hydroxide (CH), Vitapex® (VP), linezolid (LZ), a combination of LZ with CH (LC) against Enterococcus faecalis (EF). STUDY DESIGN: EF strains were mixed with peptone water and the turbidity was adjusted to the McFarland's turbidity standard tube No: 0.5. The inoculum obtained was used to make lawn cultures on the agar plates. A total of 30 agar plates were prepared, such that each plate had five wells containing the five medicaments. The plates were incubated and evaluated for zones of inhibition after intervals of 24 hours and 72 hours. The results were statistically evaluated by paired t-test, ANOVA and Post-hoc analysis using Tukey's HSD. RESULTS: The difference between values of the zones of inhibition around various medicaments after 24 hours and 72 hours was found to be statistically significant. A comparison between the five groups after 24 hours or 72 hours showed that each group differed significantly from the rest of the groups. CONCLUSIONS: LC had the greatest effectiveness against EF, followed by LZ, CX, VP and CH.
PURPOSE: This study evaluated the efficacy of 2% chlorhexidine (CX), calcium hydroxide (CH), Vitapex® (VP), linezolid (LZ), a combination of LZ with CH (LC) against Enterococcus faecalis (EF). STUDY DESIGN:EF strains were mixed with peptone water and the turbidity was adjusted to the McFarland's turbidity standard tube No: 0.5. The inoculum obtained was used to make lawn cultures on the agar plates. A total of 30 agar plates were prepared, such that each plate had five wells containing the five medicaments. The plates were incubated and evaluated for zones of inhibition after intervals of 24 hours and 72 hours. The results were statistically evaluated by paired t-test, ANOVA and Post-hoc analysis using Tukey's HSD. RESULTS: The difference between values of the zones of inhibition around various medicaments after 24 hours and 72 hours was found to be statistically significant. A comparison between the five groups after 24 hours or 72 hours showed that each group differed significantly from the rest of the groups. CONCLUSIONS: LC had the greatest effectiveness against EF, followed by LZ, CX, VP and CH.