Bodal Vijay Kumar1, Kalra Ravneet2, Bal Manjit Singh3, Bhagat Ranjeev4, Gurdeep Singh Kalyan3, Gupta Nishit2, Suri Anil1. 1. Associate Professor, Department of Pathology, Government Medical College Patiala Punjab, India . 2. Junior Resident, Department of Pathology, Government Medical College Patiala Punjab, India . 3. Professor and Head, Department of Pathology, Government Medical College Patiala Punjab, India . 4. Assistant Professor, Department of Pathology, Government Medical College Patiala Punjab, India .
Abstract
INTRODUCTION: Sex chromatin is a plano-convex to triangular DNA mass measuring approximately 1μm in size and lying adjacent to the inner side of nuclear membrane in the somatic cells of the females. There is consistent loss in the sex chromatin percentage in the carcinoma cases in comparison to benign lesions and normal individuals. AIM: To know the correlation between the sex chromatin status in female breast tumors on paraffin sections, buccal smears and peripheral blood films. MATERIALS AND METHODS: The study was conducted on the paraffin sections prepared from carcinoma breast patients from their lumpectomy and mastectomy specimens. Buccal smears and a peripheral blood films were also prepared from each patient. DISCUSSION: The control group had shown a mean sex chromatin of 54.6±6.73% which was found to be similar to the mean sex chromatin percentage in the fibroadenoma breast cases i.e. 54.91±6.06%. However, the mean sex chromatin in the carcinoma breast cases was markedly reduced i.e. 8.22±6.03%. Maximum no. of fibroadenoma cases (67%) were in the younger age group i.e. 15 to 25 year, while maximum number of carcinoma breast cases (42%) occurred in the 4(th) and 5(th) decade. CONCLUSION: There is a loss of sex chromatin in cases of carcinoma breast and is associated with poor histological markers. A statistically significant correlation was also found between sex chromatin status and microscopic grading in carcinoma breast. The tumors with higher microscopic grade had lower sex chromatin as compared to those with lower microscopic grading.
INTRODUCTION: Sex chromatin is a plano-convex to triangular DNA mass measuring approximately 1μm in size and lying adjacent to the inner side of nuclear membrane in the somatic cells of the females. There is consistent loss in the sex chromatin percentage in the carcinoma cases in comparison to benign lesions and normal individuals. AIM: To know the correlation between the sex chromatin status in female breast tumors on paraffin sections, buccal smears and peripheral blood films. MATERIALS AND METHODS: The study was conducted on the paraffin sections prepared from carcinoma breastpatients from their lumpectomy and mastectomy specimens. Buccal smears and a peripheral blood films were also prepared from each patient. DISCUSSION: The control group had shown a mean sex chromatin of 54.6±6.73% which was found to be similar to the mean sex chromatin percentage in the fibroadenoma breast cases i.e. 54.91±6.06%. However, the mean sex chromatin in the carcinoma breast cases was markedly reduced i.e. 8.22±6.03%. Maximum no. of fibroadenoma cases (67%) were in the younger age group i.e. 15 to 25 year, while maximum number of carcinoma breast cases (42%) occurred in the 4(th) and 5(th) decade. CONCLUSION: There is a loss of sex chromatin in cases of carcinoma breast and is associated with poor histological markers. A statistically significant correlation was also found between sex chromatin status and microscopic grading in carcinoma breast. The tumors with higher microscopic grade had lower sex chromatin as compared to those with lower microscopic grading.
Entities:
Keywords:
Carcinoma breast; Fibroadenoma; Sex chromatin