Raghunandan Mudium1, Bhanuprakash Kolasani2. 1. Assistant Professor, Department of Pharmacology, RIMS , Raichur, India . 2. Assistant Professor, Department of Pharmacology, RIMS , Kadapa, India .
Abstract
AIMS AND OBJECTIVES: The study was aimed to evaluate the anticonvulsant effect of hydro-alcoholic seed extract of croton tiglium in rats and mice. MATERIALS AND METHODS: Forty -eight each of rats and mice of either sex were randomised into four groups and subjected to seizures induced by electroconvulsiometer and pentylenetetrazole. The hydroalcoholic seed extract of croton tiglium (250 and 500mg/kg) was studied for its anticonvulsant effect using sodium valproate (200mg/kg) as standard and distilled water as control. The parameters observed were time for onset of HLE (Hind Limb Extension) and duration of HLE in electrically induced seizures, and time for onset of convulsions and duration of convulsions in chemically induced seizures. Mortality of the animals over 24 hours was observed in both the models. For testing statistical significance between various groups unpaired student t-test was used. OBSERVATIONS AND RESULTS: In electrically induced seizures croton tiglium produced dose dependant prolongation of time for onset of HLE and a reduction in duration of HLE and in chemically induced convulsions, it prolonged time for onset of convulsions and reduced the duration of convulsions indicating its anticonvulsant effect in both models. However, anticonvulsant effect was less compared to sodium valproate. There was a higher percentage of mortality in croton tiglium group in chemically induced convulsions when compared to sodium valproate. CONCLUSION: Croton tiglium has dose dependant anticonvulsant effect in electrically induced seizures, while in pentylenetetrazole induced-seizures the protection is very minimal.
AIMS AND OBJECTIVES: The study was aimed to evaluate the anticonvulsant effect of hydro-alcoholic seed extract of croton tiglium in rats and mice. MATERIALS AND METHODS: Forty -eight each of rats and mice of either sex were randomised into four groups and subjected to seizures induced by electroconvulsiometer and pentylenetetrazole. The hydroalcoholic seed extract of croton tiglium (250 and 500mg/kg) was studied for its anticonvulsant effect using sodium valproate (200mg/kg) as standard and distilled water as control. The parameters observed were time for onset of HLE (Hind Limb Extension) and duration of HLE in electrically induced seizures, and time for onset of convulsions and duration of convulsions in chemically induced seizures. Mortality of the animals over 24 hours was observed in both the models. For testing statistical significance between various groups unpaired student t-test was used. OBSERVATIONS AND RESULTS: In electrically induced seizurescroton tiglium produced dose dependant prolongation of time for onset of HLE and a reduction in duration of HLE and in chemically induced convulsions, it prolonged time for onset of convulsions and reduced the duration of convulsions indicating its anticonvulsant effect in both models. However, anticonvulsant effect was less compared to sodium valproate. There was a higher percentage of mortality in croton tiglium group in chemically induced convulsions when compared to sodium valproate. CONCLUSION:Croton tiglium has dose dependant anticonvulsant effect in electrically induced seizures, while in pentylenetetrazole induced-seizures the protection is very minimal.