Sivasankari S1, Senthamarai S1, Anitha C1, Apurba Sankar Sastry2, Sandhya Bhatt3, Kumudhavathi M S4, Amshavathani S K5. 1. Assistant Professor, Department of Microbiology, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India . 2. Assistant Professor, Department of Microbiology, JIPMER , Pondicherry, Tamilnadu, India . 3. Assistant Professor, Department of Microbiology, PIMS , Pondicherry, Tamilnadu, India . 4. Tutor, Department of Microbiology, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India . 5. Professor & HOD, Department of Microbiology, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India .
Abstract
BACKGROUND: Tuberculosis (TB) remains a major health problem worldwide. (PTB) is commonly associated with secondary aspergilloma. Repeated exposure of Aspergillus spores can aggravate the bronchial pathology and can manifest as asthmatic episodes. AIM AND OBJECTIVE: Pulmonary invasive aspergillosis is difficult to evaluate. Culture based diagnosis is time consuming. Hence (PCR) was done to evaluate the invasive fungal aspergillosis in (PTB) patients. MATERIALS AND METHODS: Eighty sputum samples collected from TB patients were processed as per standard protocol. Species level identification was done using PCR. RESULTS: Among 80 patients, 26 isolates were obtained, only 8 were Aspergillus species. All Aspergillus were taken up for PCR evaluation. CONCLUSION: Fungal infection should be suspected in all sputum positive patients. PCR is an effective tool to diagnose invasive aspergillosis.
BACKGROUND:Tuberculosis (TB) remains a major health problem worldwide. (PTB) is commonly associated with secondary aspergilloma. Repeated exposure of Aspergillus spores can aggravate the bronchial pathology and can manifest as asthmatic episodes. AIM AND OBJECTIVE:Pulmonary invasive aspergillosis is difficult to evaluate. Culture based diagnosis is time consuming. Hence (PCR) was done to evaluate the invasive fungal aspergillosis in (PTB) patients. MATERIALS AND METHODS: Eighty sputum samples collected from TB patients were processed as per standard protocol. Species level identification was done using PCR. RESULTS: Among 80 patients, 26 isolates were obtained, only 8 were Aspergillus species. All Aspergillus were taken up for PCR evaluation. CONCLUSION:Fungal infection should be suspected in all sputum positive patients. PCR is an effective tool to diagnose invasive aspergillosis.
Authors: P Lewis White; Stéphane Bretagne; Lena Klingspor; Willem J G Melchers; Elaine McCulloch; Bettina Schulz; Niklas Finnstrom; Carlo Mengoli; Rosemary A Barnes; J Peter Donnelly; Juergen Loeffler Journal: J Clin Microbiol Date: 2010-02-10 Impact factor: 5.948
Authors: F Reichenberger; J Habicht; P Matt; R Frei; M Solèr; C T Bolliger; P Dalquen; A Gratwohl; M Tamm Journal: Bone Marrow Transplant Date: 1999-12 Impact factor: 5.483
Authors: M T Hedayati; Y Azimi; A Droudinia; B Mousavi; A Khalilian; N Hedayati; D W Denning Journal: Eur J Clin Microbiol Infect Dis Date: 2015-05-24 Impact factor: 3.267