| Literature DB >> 24780150 |
Stacey Trevathan-Tackett1, Peter Macreadie1, Peter Ralph2, Justin Seymour1.
Abstract
A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×10(5) and 8.0×10(6)cells mg(-1) DW tissue.Entities:
Keywords: Bacterial detachment; Enumeration; Flow cytometry; Microbe; Seagrass; Seagrass-associated bacteria
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Year: 2014 PMID： 24780150 DOI： 10.1016/j.mimet.2014.04.008
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363