Pablo Weilg Espejo1, Fiorella Orellana Peralta1, Hernán Cornejo Pacheres2, Luis J del Valle3, Angela Cornejo Tapia1, Jorge Bazán Mayra2, Joaquim Ruiz4, Juana Del Valle Mendoza5. 1. Faculty of Health Sciences, School of Medicine, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, Peru. 2. Dirección Regional de Salud de Cajamarca (DIRESA-Cajamarca), Cajamarca, Peru. 3. Universidad Politècnica de Catalunya (UPC), Barcelona, Spain. 4. Barcelona Centre for International Health Research (CRESIB, Hospital Clínic-Universitat de Barcelona), Barcelona, Spain. 5. Faculty of Health Sciences, School of Medicine, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, Peru Instituto de Investigación Nutricional, Lima, Peru jdelvall@upc.edu.pe.
Abstract
BACKGROUND: Gastroenteritis caused by rotavirus is responsible for approximately 810 deaths per year in children under 5 years of age in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular epidemiology of rotavirus diarrhoea in Peru. METHODS: A total of 131 stool samples were obtained from children under 5 years hospitalised from January 2010 to December 2012 in the Hospital Regional de Cajamarca (Peru). ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were performed by semi-nested multiplex RT-PCR, and sequencing was performed to confirm the PCR results. RESULTS: Of the 117 samples available, 22 (18.8%) tested positive for rotavirus by ELISA and 42 (35.9%) tested positive by RT-PCR. Among the G genotypes identified, G9 (35.7%; 15/42) and G12 (33.3%; 14/42) were the most prevalent, with the most common combination being G12/P[6] (23.8%; 10/42). CONCLUSIONS: A high prevalence of the G12/P[6] genotype was detected. It is known that this genotype is not covered by the current vaccines available. More in-depth studies are needed to determine the current rotavirus genotypes presents in Peru.
BACKGROUND:Gastroenteritis caused by rotavirus is responsible for approximately 810 deaths per year in children under 5 years of age in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular epidemiology of rotavirus diarrhoea in Peru. METHODS: A total of 131 stool samples were obtained from children under 5 years hospitalised from January 2010 to December 2012 in the Hospital Regional de Cajamarca (Peru). ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were performed by semi-nested multiplex RT-PCR, and sequencing was performed to confirm the PCR results. RESULTS: Of the 117 samples available, 22 (18.8%) tested positive for rotavirus by ELISA and 42 (35.9%) tested positive by RT-PCR. Among the G genotypes identified, G9 (35.7%; 15/42) and G12 (33.3%; 14/42) were the most prevalent, with the most common combination being G12/P[6] (23.8%; 10/42). CONCLUSIONS: A high prevalence of the G12/P[6] genotype was detected. It is known that this genotype is not covered by the current vaccines available. More in-depth studies are needed to determine the current rotavirus genotypes presents in Peru.
Authors: Tung Gia Phan; Antonio Charlys da Costa; Juana Del Valle Mendoza; Filemon Bucardo-Rivera; Johan Nordgren; Miguel O'Ryan; Xutao Deng; Eric Delwart Journal: Arch Virol Date: 2016-01-19 Impact factor: 2.574
Authors: G M Giammanco; F Bonura; F DI Bernardo; A Cascio; G Ferrera; P Dones; L Saporito; A Collura; D M Terranova; M Valenzise; M T Allù; N Casuccio; M Palermo; K Bányai; V Martella; S DE Grazia Journal: Epidemiol Infect Date: 2016-01-08 Impact factor: 4.434