L Monzote1, J Pastor2, R Scull3, L Gille4. 1. Parasitology Department, Institute of Tropical Medicine "Pedro Kouri", Havana, Cuba. Electronic address: monzote@ipk.sld.cu. 2. Parasitology Department, Institute of Tropical Medicine "Pedro Kouri", Havana, Cuba. 3. Department of Chemistry, Institute of Pharmacy and Food, Havana University, Cuba. 4. Biochemical Pharmacology and Toxicology Unit, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria.
Abstract
UNLABELLED: Chenopodium ambrosioides have been used during centuries by native people to treat parasitic diseases. AIMS OF THE STUDY: To compare the in vivo anti-leishmanial activity of the essential oil (EO) from C. ambrosioides and its major components (ascaridole, carvacrol and caryophyllene oxide). MATERIALS AND METHODS: Anti-leishmanial effect was evaluated in BALB/c mice infected with Leishmania amazonensis and treated with the EO, main compounds and artificial mix of pure components by intralesional route at 30 mg/kg every 4 days during 14 days. Diseases progression and parasite burden in infected tissues were determined. RESULTS: EO prevented lesion development compared (p<0.05) with untreated animals and treated with vehicle. In addition, the efficacy of EO was also statistically superior (p<0.05) compared with the glucantime-treated animals. No potential effects were observed with pure components treatment. Mix of pure compounds cause death of animals after 3 days of treatment. CONCLUSIONS: Our results demonstrate the superiority of EO against experimental cutaneous leishmaniasis caused by L. amazonensis.
UNLABELLED: Chenopodium ambrosioides have been used during centuries by native people to treat parasitic diseases. AIMS OF THE STUDY: To compare the in vivo anti-leishmanial activity of the essential oil (EO) from C. ambrosioides and its major components (ascaridole, carvacrol and caryophyllene oxide). MATERIALS AND METHODS: Anti-leishmanial effect was evaluated in BALB/c mice infected with Leishmania amazonensis and treated with the EO, main compounds and artificial mix of pure components by intralesional route at 30 mg/kg every 4 days during 14 days. Diseases progression and parasite burden in infected tissues were determined. RESULTS: EO prevented lesion development compared (p<0.05) with untreated animals and treated with vehicle. In addition, the efficacy of EO was also statistically superior (p<0.05) compared with the glucantime-treated animals. No potential effects were observed with pure components treatment. Mix of pure compounds cause death of animals after 3 days of treatment. CONCLUSIONS: Our results demonstrate the superiority of EO against experimental cutaneous leishmaniasis caused by L. amazonensis.
Authors: Lianet Monzote; Gerald Geroldinger; Matthias Tonner; Ramón Scull; Sritama De Sarkar; Sophie Bergmann; Markus Bacher; Katrin Staniek; Mitali Chatterjee; Thomas Rosenau; Lars Gille Journal: Phytother Res Date: 2018-04-19 Impact factor: 5.878
Authors: Laura Machín; Beatriz Tamargo; Abel Piñón; Regla C Atíes; Ramón Scull; William N Setzer; Lianet Monzote Journal: Molecules Date: 2019-11-20 Impact factor: 4.411