| Literature DB >> 24768135 |
Masayuki Takahashi1, Reyad A Elbarbary1, Norihiro Watanabe2, Atsushi Goto1, Daichi Kamiya1, Yoshihiro Watabe1, Takayoshi Uchiyama3, Miwako Narita3, Masuhiro Takahashi3, Yoshiaki Takahashi4, Noriko Ishihara5, Tatsuya Miyazawa6, Tetsuo Yoshida6, Mitsuoki Kawano7, Masato Tamura8, Masayuki Nashimoto9.
Abstract
tRNase-Z(L)-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase Z(L) that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.Entities:
Keywords: Leukemia; Multiple myeloma; RNA therapy; TRUE gene silencing; sgRNA; tRNase Z(L)
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Year: 2014 PMID： 24768135 DOI： 10.1016/j.leukres.2014.03.021
Source DB: PubMed Journal: Leuk Res ISSN： 0145-2126 Impact factor: 3.156