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Literature DB >> 24764125

Engineering the stereochemistry of cephalosporin for specific detection of pathogenic carbapenemase-expressing bacteria.

Haibin Shi¹, Yunfeng Cheng, Kyung Hyun Lee, Robert F Luo, Niaz Banaei, Jianghong Rao.

Abstract

Reported herein is the design of fluorogenic probes specific for carbapenem-resistant Enterobacteriaceae (CRE) and they were designed based on stereochemically modified cephalosporin having a 6,7-trans configuration. Through experiments using recombinant β-lactamase enzymes and live bacterial species, these probes demonstrate the potential for use in the specific detection of carbapenemases, including metallo-β-lactamases in active bacterial pathogens.

Entities: Chemical

Keywords: enzymes; fluorescence; fluorescent probes; metalloenzymes; stereochemistry

Mesh：

Substances：

Year: 2014 PMID： 24764125 DOI： 10.1002/anie.201402012

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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Cited

8 in total

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2. Performance of a Novel Fluorogenic Assay for Detection of Carbapenemase-Producing Enterobacteriaceae from Bacterial Colonies and Directly from Positive Blood Cultures.

Authors: Hoon Seok Kim; Jung Ok Kim; Ji Eun Lee; Kang Gyun Park; Hae Kyung Lee; Soo-Young Kim; Sun-Joon Min; Juhyeon Kim; Yeon-Joon Park
Journal: J Clin Microbiol Date: 2019-12-23 Impact factor: 5.948

3. Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.

Authors: Yiyan Li; Xing Yang; Weian Zhao
Journal: SLAS Technol Date: 2017-08-29 Impact factor: 3.047

4. One-Step Detection and Classification of Bacterial Carbapenemases in 10 Minutes Using Fluorescence Identification of β-Lactamase Activity.

Authors: Yanfang Feng; Joseph W R Swain; Akilan Palanisami; Shoaib Ashraf; Tayyaba Hasan
Journal: J Clin Microbiol Date: 2021-08-18 Impact factor: 5.948

Engineering the stereochemistry of cephalosporin for specific detection of pathogenic carbapenemase-expressing bacteria.

1. Quantitative detection of cells expressing BlaC using droplet-based microfluidics for use in the diagnosis of tuberculosis.

2. Performance of a Novel Fluorogenic Assay for Detection of Carbapenemase-Producing Enterobacteriaceae from Bacterial Colonies and Directly from Positive Blood Cultures.

3. Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.

4. One-Step Detection and Classification of Bacterial Carbapenemases in 10 Minutes Using Fluorescence Identification of β-Lactamase Activity.

5. Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection.

6. Intramolecular substitution uncages fluorogenic probes for detection of metallo-carbapenemase-expressing bacteria.

7. Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue.

8. A dual-caged resorufin probe for rapid screening of infections resistant to lactam antibiotics.