Chun-Hua Ma1, Rong Jiang, Jin-Duo Li, Bin Wang, Li-Wei Sun, Yuan Lv. 1. Department of Tumor Intervention, Key Laboratory of Cerebrovascular and Neurodegenerative Diseases in Tianjin, Tianjin, China E-mail : jiangrong198@sina.com.
Abstract
OBJECTIVE: To explore the mechanism and significance of tumor angiogenesis by observing changes of micro-vessel density (MVD) and expression of vascular endothelial growth factor (VEGF) in residual tumor tissues after cryoablation. MATERIALS AND METHODS: A total of 18 nude mice xenograft models with transplanted lung adenocarcinoma cell line A549 were established and randomly divided into 3 groups when the maximum diameter of tumor reached 1 cm: control, cisplatin (DDP) and cryoablation. The nude mice were sacrificed after 21-d cryoablation to obtain the tumor tissues. Then immunohistochemistry was applied to determine MVD and the expression of VEGF in tumor tissues. RESULTS: The tumor volumes of control group, DDP group and cryoablation group were 1.48 ± 0.14 cm3, 1.03 ± 0.12 cm3 and 0.99 ± 0.06 cm3 respectively and the differences were significant (P < 0.01), whereas MVD values were 21.1 ± 0.86, 24.7 ± 0.72 and 29.2 ± 0.96 (P < 0.01) and the positive expression rates of VEGF were 36.2 ± 1.72%, 39.0 ± 1.79% and 50.8 ± 2.14% (P < 0.01), respectively, showing that MVD was proportional to the positive expression of VEGF (r = 0.928, P < 0.01). CONCLUSIONS: Cryoablation can effectively inhibit tumor growth, but tumor angiogenesis significantly increases in residual tumors, with high expression of VEGF playing an important role in the residual tumor angiogenesis.
OBJECTIVE: To explore the mechanism and significance of tumor angiogenesis by observing changes of micro-vessel density (MVD) and expression of vascular endothelial growth factor (VEGF) in residual tumor tissues after cryoablation. MATERIALS AND METHODS: A total of 18 nude mice xenograft models with transplanted lung adenocarcinoma cell line A549 were established and randomly divided into 3 groups when the maximum diameter of tumor reached 1 cm: control, cisplatin (DDP) and cryoablation. The nude mice were sacrificed after 21-d cryoablation to obtain the tumor tissues. Then immunohistochemistry was applied to determine MVD and the expression of VEGF in tumor tissues. RESULTS: The tumor volumes of control group, DDP group and cryoablation group were 1.48 ± 0.14 cm3, 1.03 ± 0.12 cm3 and 0.99 ± 0.06 cm3 respectively and the differences were significant (P < 0.01), whereas MVD values were 21.1 ± 0.86, 24.7 ± 0.72 and 29.2 ± 0.96 (P < 0.01) and the positive expression rates of VEGF were 36.2 ± 1.72%, 39.0 ± 1.79% and 50.8 ± 2.14% (P < 0.01), respectively, showing that MVD was proportional to the positive expression of VEGF (r = 0.928, P < 0.01). CONCLUSIONS: Cryoablation can effectively inhibit tumor growth, but tumor angiogenesis significantly increases in residual tumors, with high expression of VEGF playing an important role in the residual tumor angiogenesis.
Authors: Alp Tuna Beksac; Gerant Rivera-Sanfeliz; Catherine A Dufour; Unwanaobong Nseyo; Zachary Hamilton; Sean W Berquist; Abd-elRahman Hassan; Omer A Raheem; Song Wang; Robert W Wake; Robert E Gold; Ithaar H Derweesh Journal: World J Urol Date: 2016-08-02 Impact factor: 4.226